製品の概要
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製品名Anti-BCAR1 (phospho Y751) antibody
BCAR1 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to BCAR1 (phospho Y751)
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特異性The antibody detects a 130 kDa protein corresponding to the molecular mass of phosphorylated p130 Cas on SDS-PAGE immunoblots of pervanadate treated human endothelial and A431 cells, but is not observed in control cells.
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アプリケーション適用あり: WB, ELISAmore details
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種交差性交差種: Rat
交差が予測される動物種: Mouse, Human
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免疫原
Phospho-p130 Cas (Tyr-751) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 751 in rat p130 Cas. .
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ポジティブ･コントロール
- Pervanadate treated human endothelial and A431 cells
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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バッファーPreservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1mg/ml BSA -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to phospho-p130 Cas (Tyr-762) peptide before affinity purification using phospho-p130 Cas (Tyr-751) peptide (without carrier).
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab45486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/2000. Predicted molecular weight: 93 kDa. | |
| ELISA | 1/2000. |
ターゲット情報
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機能Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.
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組織特異性Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.
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配列類似性Belongs to the CAS family.
Contains 1 SH3 domain. -
ドメインContains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL1 SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9.
A serine-rich region promotes activation of the serum response element (SRE).
The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK11. -
翻訳後修飾PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix.
Dephosphorylated by PTPN14 at Tyr-128. -
細胞内局在Cell junction, focal adhesion. Cytoplasm. Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation.
- Information by UniProt
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参照データベース
- Entrez Gene: 9564 Human
- Entrez Gene: 12927 Mouse
- Entrez Gene: 25414 Rat
- Omim: 602941 Human
- SwissProt: P56945 Human
- SwissProt: Q61140 Mouse
- SwissProt: Q63767 Rat
- Unigene: 479747 Human
see all -
別名
- BCAR 1 antibody
- Bcar1 antibody
- BCAR1_HUMAN antibody
see all
Anti-BCAR1 (phospho Y751) antibody 画像
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Western blot - BCAR1 (phospho Y751) antibody (ab45486)Lanes 1 - 2 : anti-p130 Cas
Lanes 3 - 4 : Anti-BCAR1 (phospho Y751) antibody (ab45486) at 1/2000 dilution
Lanes 5 - 6 : anti-p130 Cas (Tyr-762)
Lane 1 : human endothelial cells serum starved overnight
Lane 2 : human endothelial cells treated with pervanadate (1 mM) for 30 minutes
Lane 3 : human endothelial cells serum starved overnight
Lane 4 : human endothelial cells treated with pervanadate (1 mM) for 30 minutes
Lane 5 : human endothelial cells serum starved overnight
Lane 6 : human endothelial cells treated with pervanadate (1 mM) for 30 minutes
Predicted band size : 93 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?)
プロトコール
Anti-BCAR1 (phospho Y751) antibody (ab45486) 使用論文
ab45486 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"