Anti-BCAR1 (phospho Y751) 抗体 (ab45486)

製品の概要

• 製品名Anti-BCAR1 (phospho Y751) antibody
  BCAR1 一次抗体 製品一覧
• 製品の詳細
  Rabbit polyclonal to BCAR1 (phospho Y751)
• 特異性The antibody detects a 130 kDa protein corresponding to the molecular mass of phosphorylated p130 Cas on SDS-PAGE immunoblots of pervanadate treated human endothelial and A431 cells, but is not observed in control cells.
• アプリケーション適用あり: WB, ELISAmore details
• 種交差性
  交差種: Rat
  交差が予測される動物種: Mouse, Human
• 免疫原

  Phospho-p130 Cas (Tyr-751) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 751 in rat p130 Cas. .

• ポジティブ･コントロール
  • Pervanadate treated human endothelial and A431 cells

製品の特性

• 製品の状態Liquid
• 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
• バッファーPreservative: 0.05% Sodium Azide
  Constituents: 50% Glycerol, PBS, 1mg/ml BSA
• Concentration information loading...
• 精製度Immunogen affinity purified
• 特記事項（精製）This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to phospho-p130 Cas (Tyr-762) peptide before affinity purification using phospho-p130 Cas (Tyr-751) peptide (without carrier).
• ポリ/モノポリクローナル
• アイソタイプIgG
• 研究分野
  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Other

  • Cancer
  • Signal transduction
  • Other

関連製品

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

アプリケーション

ターゲット情報

• 機能Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.
• 組織特異性Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.
• 配列類似性Belongs to the CAS family.
  Contains 1 SH3 domain.
• ドメインContains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL1 SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9.
  A serine-rich region promotes activation of the serum response element (SRE).
  The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK11.
• 翻訳後修飾PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix.
  Dephosphorylated by PTPN14 at Tyr-128.
• 細胞内局在Cell junction, focal adhesion. Cytoplasm. Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation.

• Target information above from: UniProt accession P56945 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 参照データベース
  • Entrez Gene: 9564 Human
  • Entrez Gene: 12927 Mouse
  • Entrez Gene: 25414 Rat
  • Omim: 602941 Human
  • SwissProt: P56945 Human
  • SwissProt: Q61140 Mouse
  • SwissProt: Q63767 Rat
  • Unigene: 479747 Human

  • Unigene: 3758 Mouse
  • Unigene: 40101 Rat

  see all
• 別名
  • BCAR 1 antibody
  • Bcar1 antibody
  • BCAR1_HUMAN antibody

  • Breast cancer anti estrogen resistance 1 antibody
  • Breast cancer anti estrogen resistance 1 protein antibody
  • Breast cancer anti-estrogen resistance protein 1 antibody
  • CAS antibody
  • Cas scaffolding protein family member 1 antibody
  • CAS1 antibody
  • Cass1 antibody
  • Crk associated substrate antibody
  • Crk associated substrate p130Cas antibody
  • CRK-associated substrate antibody
  • CRKAS antibody
  • FLJ12176 antibody
  • FLJ45059 antibody
  • p130cas antibody

  see all