製品の概要

  • 製品名Anti-BCAR1 (phospho Y751) antibody
    BCAR1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to BCAR1 (phospho Y751)
  • 特異性The antibody detects a 130 kDa protein corresponding to the molecular mass of phosphorylated p130 Cas on SDS-PAGE immunoblots of pervanadate treated human endothelial and A431 cells, but is not observed in control cells.
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Rat
    交差が予測される動物種: Mouse, Human
  • 免疫原

    Phospho-p130 Cas (Tyr-751) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 751 in rat p130 Cas. .

  • ポジティブ・コントロール
    • Pervanadate treated human endothelial and A431 cells

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS, 1mg/ml BSA
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to phospho-p130 Cas (Tyr-762) peptide before affinity purification using phospho-p130 Cas (Tyr-751) peptide (without carrier).
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab45486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Predicted molecular weight: 93 kDa.
ELISA 1/2000.

ターゲット情報

  • 機能Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.
  • 組織特異性Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.
  • 配列類似性Belongs to the CAS family.
    Contains 1 SH3 domain.
  • ドメインContains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL1 SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9.
    A serine-rich region promotes activation of the serum response element (SRE).
    The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK11.
  • 翻訳後修飾PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix.
    Dephosphorylated by PTPN14 at Tyr-128.
  • 細胞内局在Cell junction, focal adhesion. Cytoplasm. Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BCAR 1 antibody
    • Bcar1 antibody
    • BCAR1_HUMAN antibody
    • Breast cancer anti estrogen resistance 1 antibody
    • Breast cancer anti estrogen resistance 1 protein antibody
    • Breast cancer anti-estrogen resistance protein 1 antibody
    • CAS antibody
    • Cas scaffolding protein family member 1 antibody
    • CAS1 antibody
    • Cass1 antibody
    • Crk associated substrate antibody
    • Crk associated substrate p130Cas antibody
    • CRK-associated substrate antibody
    • CRKAS antibody
    • FLJ12176 antibody
    • FLJ45059 antibody
    • p130cas antibody
    see all

Anti-BCAR1 (phospho Y751) antibody 画像

  • Lanes 1 - 2 : anti-p130 Cas
    Lanes 3 - 4 : Anti-BCAR1 (phospho Y751) antibody (ab45486) at 1/2000 dilution
    Lanes 5 - 6 : anti-p130 Cas (Tyr-762)

    Lane 1 : human endothelial cells serum starved overnight
    Lane 2 : human endothelial cells treated with pervanadate (1 mM) for 30 minutes
    Lane 3 : human endothelial cells serum starved overnight
    Lane 4 : human endothelial cells treated with pervanadate (1 mM) for 30 minutes
    Lane 5 : human endothelial cells serum starved overnight
    Lane 6 : human endothelial cells treated with pervanadate (1 mM) for 30 minutes


    Predicted band size : 93 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)

Anti-BCAR1 (phospho Y751) antibody (ab45486) 使用論文

ab45486 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab45486.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"