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Our Abpromise guarantee covers the use of ab112521 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 13 kDa. Recombinant tested only. The detection limit for BCA1 is approximately 2.5ng/lane under non-reducing and reducing conditions.|
|ICC/IF||Use at an assay dependent concentration.|
ab112521 staining BCA1 in Rat brain tissue sections by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 1% in PBS and blocked with 2% BSA for 20 minutes at 21°C. Samples were incubated with primary antibody (1/1000 in PBS-T) for 12 hours. An Alexa Fluor®594-conjugated Donkey anti-rabbit polyclonal(1/1000) was used as the secondary antibody.