Anti-Bax inhibitor 1 抗体 (ab18852)

製品の概要

  • 製品名Anti-Bax inhibitor 1 antibody
    Bax inhibitor 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Bax inhibitor 1
  • 特異性ab18852 recognises Bax inhibitor.
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (Human) of Bax inhibitor 1.

  • ポジティブ・コントロール
    • rat testis tissue lysate

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.02% Thimerosal (merthiolate)
    Constituents: 50% Glycerol, 1% BSA, PBS, pH 7.2
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab18852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 26 kDa.

ターゲット情報

Anti-Bax inhibitor 1 antibody 画像

  • Anti-Bax inhibitor 1 antibody (ab18852) at 1 µg/ml + rat testis tissue lysate

    Predicted band size : 26 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab18852 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18852, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Bax inhibitor 1 antibody (ab18852) 使用論文

ab18852 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.
I am sorry the antibody did not give the expected results following the suggestions.
I am happy to send a free of charge replacement as it is still covered by the Abpromise guarantee. Unfortunately, the only lot avai...

Read More

I am sorry to hear you have been experiencing problems with one of our antibodies. The quality of our products is important to us and I would like to reassure you that we investigate all customer complaints. I appreciate the time taken to submit this i...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"