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RabMAb

Anti-Bax 抗体 [SP47] (ab81083)

製品の概要

  • 製品名
    Anti-Bax antibody [SP47]
    Bax 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [SP47] to Bax
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: Flow Cyt, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow
  • 免疫原

    Synthetic peptide within Human Bax aa 50-150 (internal sequence). The exact sequence is proprietary.
    Database link: Q07812

  • ポジティブ・コントロール
    • IHC-P: Human breast, cervix, esophagus, prostate, skin, tonsil, skin squamous cell carcinoma, lung squamous cell carcinoma and HK lymphoma tissues. Flow Cyt: HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab81083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/100.

Primary antibody incubation for 30 minutes at 4°C.

IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary antibody incubation for 30 minutes at room temperature.

ターゲット情報

  • 機能
    Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
  • 組織特異性
    Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.
  • 配列類似性
    Belongs to the Bcl-2 family.
  • ドメイン
    Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • 細胞内局在
    Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Apoptosis regulator BAX antibody
    • BAX antibody
    • Bax-protein antibody
    • BAX_HUMAN antibody
    • BAXA antibody
    • Baxdelta2G9 antibody
    • Baxdelta2G9omega antibody
    • Baxdelta2omega antibody
    • Bcl-2-like protein 4 antibody
    • BCL2 associated X protein antibody
    • BCL2 associated X protein omega antibody
    • BCL2 associated X protein transcript variant delta2 antibody
    • Bcl2-L-4 antibody
    • BCL2L4 antibody
    • membrane isoform alpha antibody
    see all

画像

  • Flow cytometric analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, labeling Bax with ab81083 at 1/100 dilution (green) compared to a negative control of rabbit IgG (blue).

     

  • Formalin-fixed, paraffin-embedded human esophagus tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human prostate tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human breast tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human breast tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human cervix tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human skin tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for Bax using ab81083 at 1/50 dilution in immunohistochemical analysis.

参考文献

ab81083 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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