製品の概要

  • 製品名Anti-Bax antibody
    Bax 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Bax
  • アプリケーション適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Human, Recombinant Fragment
  • 免疫原

    Recombinant full length protein (proprietary-tag) corresponding to Human Bax aa 1-192.
    Sequence:

    MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPV PQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMF SDGNFNWGRVVALFYFASKLVLKALCTKVPELIRTIMGWTLDFLRERLLG WIQDQGGWDGLLSYFGTPTWQTATIFVAGVLTASLTIWKKMG


    Database link: Q07812

  • ポジティブ・コントロール
    • Human placenta tissue; HeLa cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab54829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 3 µg/ml.
WB Use a concentration of 1 - 5 µg/ml.

This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein

ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
  • 組織特異性Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.
  • 配列類似性Belongs to the Bcl-2 family.
  • ドメインIntact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • 細胞内局在Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Apoptosis regulator BAX antibody
    • BAX antibody
    • Bax-protein antibody
    • BAX_HUMAN antibody
    • BAXA antibody
    • Baxdelta2G9 antibody
    • Baxdelta2G9omega antibody
    • Baxdelta2omega antibody
    • Bcl-2-like protein 4 antibody
    • BCL2 associated X protein antibody
    • BCL2 associated X protein omega antibody
    • BCL2 associated X protein transcript variant delta2 antibody
    • Bcl2-L-4 antibody
    • BCL2L4 antibody
    • membrane isoform alpha antibody
    see all

Anti-Bax antibody 画像

  • Immunofluorescence analysis of HeLa cells, staining Bax with ab54829 at 10 µg/ml

  • Anti-Bax antibody (ab54829) at 1 µg/ml + Recombinant tagged full length Bax protein
  • Immunohisotchemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue, staining Bax with ab54829 at 3 µg/ml.

Anti-Bax antibody (ab54829) 使用論文

ab54829 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"