製品の概要

  • 製品名Anti-Bax antibody
    Bax 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Bax
  • 特異性AHP471 recognises the human BAX protein, a proapoptotic member of the Bcl2 family.
  • アプリケーション適用あり: IHC-P, WB, IPmore details
    適用なし: ICC/IF
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide corresponding to Human Bax aa 43-61 (internal sequence).
    Sequence:

    CPELALDPVPQDASTKKLSE


    Database link: Q07812

  • 特記事項

    This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab10813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 21 kDa.
IP 1/1000.
  • 追加情報Is unsuitable for ICC/IF.
  • ターゲット情報

    • 機能Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
    • 組織特異性Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.
    • 配列類似性Belongs to the Bcl-2 family.
    • ドメインIntact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
    • 細胞内局在Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Apoptosis regulator BAX antibody
      • BAX antibody
      • Bax-protein antibody
      • BAX_HUMAN antibody
      • BAXA antibody
      • Baxdelta2G9 antibody
      • Baxdelta2G9omega antibody
      • Baxdelta2omega antibody
      • Bcl-2-like protein 4 antibody
      • BCL2 associated X protein antibody
      • BCL2 associated X protein omega antibody
      • BCL2 associated X protein transcript variant delta2 antibody
      • Bcl2-L-4 antibody
      • BCL2L4 antibody
      • membrane isoform alpha antibody
      see all

    Anti-Bax antibody 画像

    • ab10813 immunoprecipitating Bax. 2mg of human neuroblastoma whole cell lysate was incubated with ab10813 at a 1/1000 dilution for 16 hours and then precipitated with protein A. An HRP polyclonal antibody was used as the secondary antibody.

      This image is courtesy of an Abreview submitted by David MacDonald on 6 February 2006.

      See Abreview

    Anti-Bax antibody (ab10813) 使用論文

    This product has been referenced in:

    See all 5 Publications for this product

    Product Wall

    Thank you for your inquiry.


    We have two antibodies tested in Bax in ICC/IF as ab10813 and ab62641.


    http://www.abcam.com/index.html?datasheet=10813 (or use the following: http://www.abcam.com/index.html?datasheet=10813). ...

    Read More

    Thank you for your inquiry. This antibody will recognize both native and activated BAX. I hope this information helps. Please let me know if you have any additional questions.

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% Triton X100 in PBS
    Username

    Dr. Kirk McManus

    Verified customer

    投稿 Mar 07 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunoprecipitation
    Sample Human Cell lysate - whole cell (Neuroblastoma)
    Specification Neuroblastoma
    Immuno-precipitation step Protein A
    Username

    Mr. David MacDonald

    Verified customer

    投稿 Feb 06 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"