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製品の概要

  • 製品名Anti-Bax antibody [2D2]
    Bax 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2D2] to Bax
  • 特異性The immunogen amino acid sequence is NOT shared by mouse and rat bax protein.
  • アプリケーション適用あり: Flow Cyt, Sandwich ELISA, WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cow, Cat, Dog, Chimpanzee, Macaque monkey非交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human Bax aa 3-16 (Cysteine residue).
    Sequence:

    C-GSGEQPRGGGPTSS


    Database link: Q07812

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ･コントロール
    • HeLa cells. Hodgkin’s lymphoma. ICC/IF: A549 cells
  • 特記事項

    This antibody is BSA and azide free

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Sandwich ELISA Use a concentration of 0.2 µg/ml. Can be paired for Sandwich ELISA with Rabbit monoclonal [E63] to Bax (ab32503).
WB Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
  • 組織特異性Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.
  • 配列類似性Belongs to the Bcl-2 family.
  • ドメインIntact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • 細胞内局在Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.
  • Information by UniProt
  • 参照データベース
    • 別名
      • Apoptosis regulator BAX antibody
      • BAX antibody
      • Bax-protein antibody
      • BAX_HUMAN antibody
      • BAXA antibody
      • Baxdelta2G9 antibody
      • Baxdelta2G9omega antibody
      • Baxdelta2omega antibody
      • Bcl-2-like protein 4 antibody
      • BCL2 associated X protein antibody
      • BCL2 associated X protein omega antibody
      • BCL2 associated X protein transcript variant delta2 antibody
      • Bcl2-L-4 antibody
      • BCL2L4 antibody
      • membrane isoform alpha antibody
      see all

    Anti-Bax antibody [2D2] 画像

    • Immunocytochemistry/ Immunofluorescence - Anti-Bax antibody [2D2] (ab77566)
      Immunocytochemistry/ Immunofluorescence - Anti-Bax antibody [2D2] (ab77566)

      ab77566 staining Bax in A549 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab77566 at a working concentration of 5μg/ml and ab202272, Rabbit monoclonal [EP1332Y] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 1/250 overnight at +4°C, followed by a further incubation at room temperature for 1h with an anti-mouse AlexaFluor® 488 (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Western blot - Anti-Bax [2D2] antibody (ab77566)
      Western blot - Anti-Bax [2D2] antibody (ab77566)


      Predicted band size : 21 kDa
      Lane 1 = Bax protein (Tagged) (ab85157) ? 10 ng. Lane 2 = Extract of HeLa cells ? 40 ug. Lane 3 = Extract of HepG2 cells ? 40 ug. Lane 4 = Bax protein (Tagged) (ab85157) ? 10 ng. Lane 5 = Extract of HeLa cells ? 40 ug. Lane 6 = Extract of HepG2 cells ? 40 ug. SDS PAGE performed under reducing conditions (100mM DTT ? Sample heated at 50?C). Primary : Lanes 1-3: Anti Bax antibody (ab77566) at 1 ug/mL. Lanes 4-6: Anti Bax antibody (ab32503) at 1:2000 dilution. Secondary : Lanes 1-3: Goat anti mouse IgG(H&L)-HRP at 1:10000. Lanes 4-6: Goat anti rabbit IgG(H&L)-HRP at 1:10000. Development: ECL with 2 min exposure. Blocking: in 5% Milk + PBS for 3 hours at RT. Primary antibody: in 5% Milk + PBS overnight at 4 C. Secondary antibody: in 5% Milk + PBS for 2 hour at RT. Predicted band size : Bax 21kDa and Bax (Tagged) 49 kDa. Observed band size : Bax 21kDa and Bax (Tagged) 49 kDa
    • Western blot - Anti-Bax antibody [2D2] - BSA and Azide free (ab77566)
      Western blot - Anti-Bax antibody [2D2] - BSA and Azide free (ab77566)
      Anti-Bax antibody [2D2] (ab77566) at 1 µg/ml + HeLa cell lysate

      Predicted band size : 21 kDa
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [2D2] - BSA and Azide free (ab77566)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [2D2] - BSA and Azide free (ab77566)

      Immunohistochemical analysis of Human Hodgkin's lymphoma tissue, staining Bax with ab77566 at 4 µg/ml.

    • Flow Cytometry - Anti-Bax antibody [2D2] - BSA and Azide free (ab77566)
      Flow Cytometry - Anti-Bax antibody [2D2] - BSA and Azide free (ab77566)

      Overlay histogram showing HeLa cells stained with ab77566 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77566, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    • Sandwich ELISA - Anti-Bax antibody [2D2] (ab77566)
      Sandwich ELISA - Anti-Bax antibody [2D2] (ab77566)
      Standard Curve for Bax (Analyte: ab85157) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [2D2] to Bax - BSA and Azide free (ab77566) at 0.2ug/ml and Detector Antibody Rabbit monoclonal [E63] to Bax (ab32503) at 0.5ug/ml Concentration of ab32503 may vary from lot to lot; please use this curve as guideline.

    プロトコール

    Anti-Bax antibody [2D2] (ab77566) 使用論文

    This product has been referenced in:
    • Wang H  et al. Nedaplatin sensitization of cisplatin-resistant human non-small cell lung cancer cells. Oncol Lett 11:2566-2572 (2016). WB ; Human . Read more (PubMed: 27073518) »
    • Arici S  et al. Central nervous system toxicity after acute oral formaldehyde exposure in rabbits: An experimental study. Hum Exp Toxicol N/A:N/A (2014). IHC ; Rabbit . Read more (PubMed: 24501105) »

    See all 3 Publications for this product

    ab77566 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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