製品の概要

  • 製品名
    Anti-Bassoon antibody [SAP7F407]
    Bassoon 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [SAP7F407] to Bassoon
  • アプリケーション
    適用あり: WB, IP, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    Recombinant rat Bassoon protein

  • ポジティブ・コントロール
    • Rat brain tissue extract This antibody gave a positive result in IHC in the following FFPE tissue: Human normal cerebral cortex.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    SAP7F407
  • アイソタイプ
    IgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab82958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Predicted molecular weight: 416 kDa.
IP Use at an assay dependent concentration.
ICC/IF 1/400.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Is thought to be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. Seems to act through binding to ERC2/CAST1. Essential in regulated neurotransmitter release from a subset of brain glutamatergic synapses. Involved in the formation of the retinal photoreceptor ribbon synapses.
  • 組織特異性
    Exclusively expressed in brain.
  • 翻訳後修飾
    Myristoylated. The N-terminal myristoylation is not sufficient for presynaptic localization.
  • 細胞内局在
    Cytoplasm. Cell junction > synapse > synaptosome. Cytoplasm > cytoskeleton. Localized to the active zone of presynaptic density.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Bassoon protein antibody
    • bsn antibody
    • BSN_HUMAN antibody
    • KIAA0434 antibody
    • Neuronal double zinc finger protein antibody
    • Presynaptic cytomatrix protein antibody
    • Protein bassoon antibody
    • Zinc finger protein 231 antibody
    • ZNF 231 antibody
    • ZNF231 antibody
    see all

画像

  • IHC-P image of Bassoon staining on mouse cerebellum sections using ab82958 (1:1500). The sections were deparaffinized and subjected to heat mediated antigen retrieval using HEIR. The sections were blocked using 1% BSA for 10 mins at 21°C. ab82958 was diluted 1:1500 and incubated with the sctions for 2 hours at 21°C. The secondary antibody used was goat polyclonal to mouse IgG conjugated to Biotin (1:200).

  • Anti-Bassoon antibody [SAP7F407] (ab82958) at 1/500 dilution + rat brain tissue extract
    Developed using the ECL technique

    Predicted band size : 416 kDa
  • Bassoon localization in the rat brain cerebellar molecular layer using Bassoon monoclonal antibody (ab82958).
  • IHC-P image of Bassoon staining on rat cerebellum sections using ab82958 (1:1000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab82958 was diluted 1:1000 and incubated with the sections for 2 hours at 21°C. The secondary antibody used was goat polyclonal to mouse IgG conjugated to biotin (1:200).

  • IHC image of Bassoon staining in Human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82958, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

参考文献

This product has been referenced in:

See all 19 Publications for this product

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rabbit Cell (Retina Section)
Permeabilization
Yes - Triton X-100
Specification
Retina Section
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jun 22 2017

Application
Flow Cytometry
Sample
Human Cell (Differentiated hNSCs)
Permeabilization
Yes - 0.25% Triton X-100 in DPBS
Gating Strategy
Undifferentiated Stem Cells (white)
Specification
Differentiated hNSCs
Preparation
Cell harvesting/tissue preparation method: Accutase
Sample buffer: PBS
Fixation
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 24 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Zebra finch Tissue sections (Area X (striatum) brain tissue)
Antigen retrieval step
None
Permeabilization
Yes - PBST
Specification
Area X (striatum) brain tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Mr. Jonnathan Singh

Verified customer

投稿 May 19 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Sample
Rat Cell (hippocampal neuron)
Specification
hippocampal neuron
Permeabilization
Yes - 0.2% TritonX
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jan 31 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Marmoset (common) Tissue sections (Cerebellum)
Specification
Cerebellum
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Dec 04 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Goat Tissue sections (Cerebellum)
Specification
Cerebellum
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Dec 04 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: HIER
Sample
Mouse Tissue sections (Cerebellum)
Specification
Cerebellum
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Oct 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Rat Tissue sections (Cerebellum)
Specification
Cerebellum
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Sep 27 2013

Thank you for contacting us.

This antibody was raised against recombinant rat Bassoon protein and it has not been epitope mapped. It will have one specific binding epitope, however that region has not been determined.

I am sorry t...

Read More

Thank you for contacting us.

We have 4 antibodies in our catalog raised against Bassoon: ab82958 (mouse), ab76065 (rabbit), ab110426 (rabbit) and ab101772 (goat).

Could you please let me know what kind of IHC you are planning to pe...

Read More

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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