製品の概要

  • 製品名BAPTA-AM
  • 製品の詳細
    Selective Ca2+ chelator. Analog of BAPTA.
  • 生理活性の詳細Selective Ca2+ chelator. Cell-permeable analog of BAPTA (ab120449). Useful for manipulation of intracellular free Ca2+ levels. Shows varied biological activity. Blocks hKv1.5, Kv11.1 (hERG) and hKv1.3 channels (Ki values are 1.23, 1.30 and 1.45 μM, respectively).
  • 精製度> 98%

製品の特性

    BAPTA-AM 画像

    • ab66705 staining PAI1 in HepG2 cells treated with BAPTA-AM (ab120503), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of BAPTA-AM, as described in literature.
      The cells were incubated at 37°C for 4 hours in media containing different concentrations of ab120503 (BAPTA-AM) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    BAPTA-AM (ab120503) 使用論文

    This product has been referenced in:
    • Chi Y  et al. Nonsteroidal anti-inflammatory drug flufenamic acid is a potent activator of AMP-activated protein kinase. J Pharmacol Exp Ther 339:257-66 (2011). Read more (PubMed: 21765041) »
    • Tang Q  et al. The membrane permeable calcium chelator BAPTA-AM directly blocks human ether a-go-go-related gene potassium channels stably expressed in HEK 293 cells. Biochem Pharmacol 74:1596-607 (2007). Read more (PubMed: 17826747) »
    • Kowaltowski AJ  et al. Elevation of resting mitochondrial membrane potential of neural cells by cyclosporin A, BAPTA-AM, and bcl-2. Am J Physiol Cell Physiol 279:C852-9 (2000). Read more (PubMed: 10942734) »

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