ICC/IF image of ab37120 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab37120 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-BAP31 antibody (ab37120)
Lane 1 : Anti-BAP31 antibody (ab37120) at 0.5 µg/ml Lane 2 : Anti-BAP31 antibody (ab37120) at 1 µg/ml Lane 3 : Anti-BAP31 antibody (ab37120) at 2 µg/ml
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