Customer reviews and FAQs

製品の概要

  • 製品名
    Anti-BAP31 antibody [7A3BB6]
    BAP31 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [7A3BB6] to BAP31
  • 特異性
    Shotgun immunization of human HeLa cell lysates into mice. Targets were determined by mass spectrometry and validated by WB, ICC, ELISA pair and other techniques.
  • アプリケーション
    適用あり: IHC-P, ICC/IF, WB, IP, In-Cell ELISA, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Hela cell fraction

  • ポジティブ･コントロール
    • HepG2 cell lysate; HL60 and Human fibroblast cells. Human normal colon FFPE tissue.
  • 特記事項

    Product was previously marketed under the MitoSciences sub-brand.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • バッファー
    Preservative: 0.02% Sodium azide
    Constituent: 99.98% HBS
  • Concentration information loading...
  • 精製度
    >95% by SDS-PAGE
  • 特記事項（精製）
    ab112993 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Monoclonal purity was near homogeneity as judged by SDS-PAGE.
  • ポリ/モノ
    モノクローナル
  • クローン名
    7A3BB6
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab112993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 28 kDa.
IP Use at an assay dependent concentration.
In-Cell ELISA Use a concentration of 1 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    May play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi. May be involved in CASP8-mediated apoptosis.
  • 組織特異性
    Ubiquitous.
  • 関連疾患
    Note=BCAP31 is deleted in the chromosome Xq28 deletion syndrome which involves BCAP31 and the and the promoter region of ABCD1.
  • 配列類似性
    Belongs to the BCAP29/BCAP31 family.
  • 翻訳後修飾
    Cleaved by CASP8 and other caspases.
  • 細胞内局在
    Endoplasmic reticulum membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. May shuttle between the ER and the intermediate compartment/cis-Golgi complex.
  • Information by UniProt
  • 参照データベース
    • 別名
      • 6C6 AG antibody
      • 6C6 AG tumor associated antigen antibody
      • 6C6-AG tumor-associated antigen antibody
      • 6C6AG antibody
      • 6C6AG tumor associated antigen antibody
      • Accessory protein BAP 31 antibody
      • Accessory protein BAP31 antibody
      • B cell receptor associated protein 31 antibody
      • B-cell receptor-associated protein 31 antibody
      • BA31 antibody
      • BAP 31 antibody
      • Bap31 antibody
      • BAP31_HUMAN antibody
      • BCAP 31 antibody
      • BCAP31 antibody
      • BCR associated protein Bap 31 antibody
      • BCR associated protein Bap31 antibody
      • BCR-associated protein 31 antibody
      • CDM antibody
      • CDM protein antibody
      • DXS1357E antibody
      • MS950 antibody
      • p28 antibody
      • p28 Bap31 antibody
      • Protein CDM antibody
      • RP23-329M9.5 antibody
      see all

    Anti-BAP31 antibody [7A3BB6] 画像

    • Western blot - BAP31 antibody [7A3BB6] (ab112993)
      Western blot - BAP31 antibody [7A3BB6] (ab112993)
      All lanes : Anti-BAP31 antibody [7A3BB6] (ab112993) at 0.5 µg/ml

      Lane 1 : HepG2 Cell Lysate
      Lane 2 : H9C2 Cell Lysate
      Lane 3 : MEF Cell Lysate


      Predicted band size : 28 kDa
    • Flow Cytometry - BAP31 antibody [7A3BB6] (ab112993)
      Flow Cytometry - BAP31 antibody [7A3BB6] (ab112993)
      ab112993 at 1ug/ml staining BAP31 in MeOH-fixed HL60 cells by Flow Cytometry (blue). Isotype control antibody (red).
    • Immunocytochemistry/ Immunofluorescence - Anti-BAP31 antibody [7A3BB6] (ab112993)
      Immunocytochemistry/ Immunofluorescence - Anti-BAP31 antibody [7A3BB6] (ab112993)
      ab112993 at 5 µg/ml staining BAP31 in Human fibroblast cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (green). The target locates mainly in the endoplasmic reticulum.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody [7A3BB6] (ab112993)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody [7A3BB6] (ab112993)

      IHC image of BAP31 staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab112993, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Anti-BAP31 antibody [7A3BB6] (ab112993) 使用論文

    ab112993 has not yet been referenced specifically in any publications.

    ab112993 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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