製品の概要

  • 製品名
    Anti-BANF2 antibody
  • 製品の詳細
    Rabbit polyclonal to BANF2
  • アプリケーション
    適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen sequence: FLSEPIGEKD VCWVDGISHE LAINLVTKGI NKAYILLGQF LLMHKNEAEF QRWLICCFGA TECEAQQTSH CLKEWCA, corresponding to amino acids 11-87 of Human BANF2.

  • ポジティブ・コントロール
    • Human pancreas tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab122806 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 4 µg/ml.

ターゲット情報

  • 機能
    May play a role in BANF1 regulation and influence tissue-specific roles of BANF1.
  • 組織特異性
    Expressed strongly in testis and pancreas. Also detected in brain, colon, liver, lung, ovary, placenta, prostate, small intestine, spleen and thymus. Not detected in heart, kidney and skeletal muscle.
  • 配列類似性
    Belongs to the BAF family.
  • 細胞内局在
    Nucleus. Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BAF-L antibody
    • BAF2 antibody
    • BAFL antibody
    • BAFL_HUMAN antibody
    • BANF2 antibody
    • Barrier-to-autointegration factor 2 antibody
    • Barrier-to-autointegration factor-like protein antibody
    • C20orf179 antibody
    see all

画像

  • Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
  • ab122806, at 1/50 dilution, staining BANF2 in paraffin-embedded Human pancreas tissue by Immunohistochemistry.
  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli using ab122806.
    Recommended treatment:
    Fixation/Permeabilization: PFA/Triton X-100

参考文献

ab122806 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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