Anti-Band 3 (phospho Y359) 抗体 [EPR1427Y] (ab77236)

製品の概要

  • 製品名Anti-Band 3 (phospho Y359) antibody [EPR1427Y]
    Band 3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR1427Y] to Band 3 (phospho Y359)
  • アプリケーション適用あり: WBmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding tyrosine 359 of human Band 3.

  • ポジティブ・コントロール
    • Red blood cell lysates treated with pervanadate
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名EPR1427Y
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab77236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50000. Predicted molecular weight: 102 kDa.
  • 追加情報Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.
    • 組織特異性Detected in erythrocytes (at protein level) (PubMed:7506871, PubMed:26542571). Isoform 2 is expressed in kidney (at protein level) (PubMed:7506871).
    • 関連疾患Ovalocytosis, Southeast Asian
      Spherocytosis 4
      Renal tubular acidosis, distal, autosomal dominant
      Renal tubular acidosis, distal, with hemolytic anemia
      Renal tubular acidosis, distal, with normal red cell morphology
      Cryohydrocytosis
    • 配列類似性Belongs to the anion exchanger (TC 2.A.31) family.
    • 翻訳後修飾Phosphorylated on Tyr-8 and Tyr-21 most likely by SYK. PP1-resistant phosphorylation that precedes Tyr-359 and Tyr-904 phosphorylation.
      Phosphorylated on Tyr-359 and Tyr-904 most likely by LYN. PP1-inhibited phosphorylation that follows Tyr-8 and Tyr-21 phosphorylation.
      N-glycosylated.
    • 細胞内局在Cell membrane. Basolateral cell membrane. Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AE 1 antibody
      • AE1 antibody
      • Anion exchange protein 1 antibody
      • Anion exchanger 1 antibody
      • B3AT_HUMAN antibody
      • Band 3 anion transport protein antibody
      • BND3 antibody
      • CD233 antibody
      • DI antibody
      • Diego blood group antibody
      • EMPB3 antibody
      • EPB3 antibody
      • Erythrocyte membrane protein band 3 antibody
      • Erythroid anion exchange protein antibody
      • FR antibody
      • Froese blood group antibody
      • RTA1A antibody
      • SLC4A1 antibody
      • Solute carrier family 4 anion exchanger member 1 antibody
      • Solute carrier family 4 member 1 antibody
      • SW antibody
      • Swann blood group antibody
      • Waldner blood group antibody
      • WD antibody
      • WD1 antibody
      • WR antibody
      • Wright blood group antibody
      see all

    Anti-Band 3 (phospho Y359) antibody [EPR1427Y] 画像

    • All lanes : Anti-Band 3 (phospho Y359) antibody [EPR1427Y] (ab77236) at 1/50000 dilution

      Lane 1 : Red blood cell lysate, untreated.
      Lane 2 : Red blood cell lysate treated with pervanadate.

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 102 kDa
      Observed band size : 102 kDa

    Anti-Band 3 (phospho Y359) antibody [EPR1427Y] (ab77236) 使用論文

    ab77236 has not yet been referenced specifically in any publications.

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