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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab32371 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 23 kDa.|
|IHC-P||Use at an assay dependent concentration.|
|Flow Cyt||1/10 - 1/20.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/250 - 1/500.|
ab32371 staining Bak in the human cell line HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permeabilized with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/20. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
Immunohistochemical analysis of Bak expression in paraffin embedded human stomach carcinoma, using 1/250 ab32371.
Bak was immunoprecipitated from HCT116 p53-/- cell line whole cell lysate with ab32371 at 1/100 dilution.
Western blot was performed from the immunoprecipitate using ab32371 at 1/2000 dilution.