Anti-Bag5 抗体 (ab97660)
Key features and details
- Rabbit polyclonal to Bag5
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Bag5 antibody -
製品の詳細
Rabbit polyclonal to Bag5 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow -
免疫原
Recombinant fragment, corresponding to a sequence within amino acids 1-165 of Human Bag5 (NP_004864).
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ポジティブ・コントロール
- 293T, HeLa, MOLT4 and Raji cell lysates; A549 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97660の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 51 kDa.
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ICC/IF |
1/100 - 1/200.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 51 kDa. |
ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
Inhibits both auto-ubiquitination of PARK2 and ubiquitination of target proteins by PARK2 (By similarity). May function as a nucleotide exchange factor for HSP/HSP70, promoting ADP release, and activating Hsp70-mediated refolding. -
配列類似性
Contains 5 BAG domains. -
ドメイン
The fifth BAG domain is responsible for the interaction with HSP70 nucleotide-binding domain. - Information by UniProt
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参照データベース
- Entrez Gene: 522854 Cow
- Entrez Gene: 9529 Human
- Entrez Gene: 70369 Mouse
- Entrez Gene: 366734 Rat
- Omim: 603885 Human
- SwissProt: Q2TA08 Cow
- SwissProt: Q9UL15 Human
- SwissProt: Q8CI32 Mouse
see all -
別名
- BAG family molecular chaperone regulator 5 antibody
- BAG-5 antibody
- BAG5 antibody
see all
画像
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All lanes : Anti-Bag5 antibody (ab97660) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : MOLT4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 51 kDa
10% SDS-PAGE. -
ab97660 at 1/200 dilution staining Bag5 in A549 cells by Immunofluorescence, Paraformaldehyde fixed. Lower image is merged with DNA probe.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab97660 は 5 報の論文で使用されています。
- Wang M et al. miRNA-770-5p expression is upregulated in patients with type 2 diabetes and miRNA-770-5p knockdown protects pancreatic ß-cell function via targeting BAG5 expression. Exp Ther Med 22:664 (2021). PubMed: 33986829
- Fan X et al. MiRNA-429 alleviates ketamine-induced neurotoxicity through targeting BAG5. Environ Toxicol 36:620-627 (2021). PubMed: 33283947
- Lin H et al. miR-4454 Promotes Hepatic Carcinoma Progression by Targeting Vps4A and Rab27A. Oxid Med Cell Longev 2021:9230435 (2021). PubMed: 34777698
- Xi J et al. miR-155 inhibition represents a potential valuable regulator in mitigating myocardial hypoxia/reoxygenation injury through targeting BAG5 and MAPK/JNK signaling. Mol Med Rep 21:1011-1020 (2020). PubMed: 31922242
- Bruchmann A et al. Bcl-2 associated athanogene 5 (Bag5) is overexpressed in prostate cancer and inhibits ER-stress induced apoptosis. BMC Cancer 13:96 (2013). WB, ICC/IF ; Human . PubMed: 23448667