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A synthetic peptide corresponding to residues in Human Bag3.
This product is a recombinant rabbit monoclonal antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab92309 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. PubMed: 24895585|
|WB||1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 62 kDa).|
|IP||1/10 - 1/100.|
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: BAG3 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: K562 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab92309 observed at 80 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab92309 was shown to recognize BAG3 in wild-type cells as signal was lost at the expected MW in BAG3 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and BAG3 knockout samples were subjected to SDS-PAGE. Ab92309 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"