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RabMAb

Anti-Bag3 抗体 [EPR3515] (ab92309)

製品の概要

  • 製品名
    Anti-Bag3 antibody [EPR3515]
    Bag3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3515] to Bag3
  • アプリケーション
    適用あり: IHC-P, WB, IPmore details
    適用なし: Flow Cyt or ICC
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human Bag3.

  • ポジティブ・コントロール
    • 293T, HeLa, MCF7 and K562 cell lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab92309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration. PubMed: 24895585
WB 1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 62 kDa).
IP 1/10 - 1/100.
  • 追加情報
    Is unsuitable for Flow Cyt or ICC.
  • ターゲット情報

    • 機能
      Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity.
    • 関連疾患
      Defects in BAG3 are the cause of myopathy myofibrillar BAG3-related (MFM-BAG3) [MIM:612954]. A neuromuscular disorder that results in early-onset, severe, progressive, diffuse muscle weakness associated with cardiomyopathy, severe respiratory insufficiency during adolescence, and a rigid spine in some patients. At ultrastructural level, muscle fibers display structural alterations consisting of replacement of the normal myofibrillar markings by small, dense granules, or larger hyaline masses, or amorphous material.
    • 配列類似性
      Contains 1 BAG domain.
      Contains 2 WW domains.
    • Information by UniProt
    • 参照データベース
    • 別名
      • BAG 3 antibody
      • BAG family molecular chaperone regulator 3 antibody
      • BAG-3 antibody
      • Bag3 antibody
      • BAG3_HUMAN antibody
      • Bcl 2 binding protein antibody
      • Bcl-2-associated athanogene 3 antibody
      • Bcl-2-binding protein Bis antibody
      • BCL2 associated athanogene 3 antibody
      • BCL2 binding athanogene 3 antibody
      • BIS antibody
      • CAIR 1 antibody
      • Docking protein CAIR 1 antibody
      • Docking protein CAIR-1 antibody
      • MFM6 antibody
      see all

    画像



    • Predicted band size : 62 kDa

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: BAG3 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: Hela whole cell lysate (20 µg)
      Lane 4: K562 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab92309 observed at 80 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab92309 was shown to recognize BAG3 in wild type cells as signal was lost at the expected MW in BAG3 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and BAG3 knockout samples were subjected to SDS-PAGE. Ab92309 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-Bag3 antibody [EPR3515] (ab92309) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 62 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)

    参考文献

    This product has been referenced in:
    • Xiao H  et al. BAG3 and HIF-1 a Coexpression Detected by Immunohistochemistry Correlated with Prognosis in Hepatocellular Carcinoma after Liver Transplantation. Biomed Res Int 2014:516518 (2014). WB, IHC-P ; Human . Read more (PubMed: 24895585) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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