製品の概要

  • 製品名Anti-BAG2 antibody
    BAG2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to BAG2
  • アプリケーション適用あり: WB, IHC-P, IHC-Fr, IP, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein (Human)

  • ポジティブ・コントロール
    • Lymphoid, and many cancer cell lines. HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab47106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP 1/50 - 1/200.
ICC/IF 1/1000 - 1/5000.

ターゲット情報

  • 機能Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release.
  • 配列類似性Contains 1 BAG domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BAG 2 antibody
    • BAG family molecular chaperone regulator 2 antibody
    • BAG-2 antibody
    • BAG-family molecular chaperone regulator-2 antibody
    • Bag2 antibody
    • BAG2_HUMAN antibody
    • BCL 2 associated athanogene 2 antibody
    • Bcl-2-associated athanogene 2 antibody
    • BCL2 associated athanogene 2 antibody
    • BCL2-associated athanogene 2 antibody
    • dJ417I1.2 (BAG family molecular chaperone regulator 2) antibody
    • dJ417I1.2 antibody
    • KIAA0576 antibody
    • MGC149462 antibody
    • OTTHUMP00000016668 antibody
    see all

Anti-BAG2 antibody 画像

  • Anti-BAG2 antibody (ab47106) at 1/2000 dilution + HeLa cells

    Predicted band size : 24 kDa
    Observed band size : 24 kDa
  • Immunofluorescence confocal microscopy of BAG-2 using ab47106 at 1:2000 on HeLa cells growing on coverslips left untreated (A) or heat shocked at 42 degrees for 30 min (B). Cells were fixed with 3.7% paraformaldehyde, permeabilized with 0.5% Triton X-100 prior to antibody staining. BAG2 localized to the cytoplasm of untreated cells, and to the nucleus of cells subjected to heat shock.

Anti-BAG2 antibody (ab47106) 使用論文

This product has been referenced in:
  • Horikawa I  et al. Autophagic degradation of the inhibitory p53 isoform ?133p53a as a regulatory mechanism for p53-mediated senescence. Nat Commun 5:4706 (2014). Read more (PubMed: 25144556) »
  • Rogon C  et al. HSP70-binding protein HSPBP1 regulates chaperone expression at a posttranslational level and is essential for spermatogenesis. Mol Biol Cell 25:2260-71 (2014). IHC-Fr ; Mouse . Read more (PubMed: 24899640) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us with your question. We only have the full size vials of each of our products, however we do guarantee that all antibodies will work in tested species and applications as stated on the datasheet for up to 6 months. So, i...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hek293T)
Loading amount 30 µg
Specification Hek293T
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Mirjam Eckert

Verified customer

投稿 Oct 29 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela)
Loading amount 40 µg
Specification Hela
Treatment lysed by RIPA w/o SDS
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 2%
Username

Mr. Jörg Hoehfeld

Verified customer

投稿 Apr 11 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"