Anti-Bag1 抗体 [Y166] (ab32109)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y166] to Bag1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Bag1 antibody [Y166]
Bag1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [Y166] to Bag1 -
由来種
Rabbit -
特異性
The antibody should recognize all three isoforms of Bag-1 (Bag-1L, Bag-1M and Bag-1S).
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アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details
適用なし: Flow Cyt -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human Bag1 aa 50-150. The exact sequence is proprietary.
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ポジティブ・コントロール
- HeLa cell lysate and cells, human prostate carcinoma tissue
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
Y166 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32109の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/500. Detects a band of approximately 33, 46, 50 kDa (predicted molecular weight: 31 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF | (1) |
1/100 - 1/250.
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特記事項 |
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WB
1/500. Detects a band of approximately 33, 46, 50 kDa (predicted molecular weight: 31 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
ターゲット情報
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機能
Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli. -
組織特異性
Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines (at protein level). -
配列類似性
Contains 1 BAG domain.
Contains 1 ubiquitin-like domain. -
翻訳後修飾
Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. -
細胞内局在
Cytoplasm. Nucleus. Isoform 2 localizes to the cytoplasm and shuttles into the nucleus in response to heat shock; Nucleus. Cytoplasm. Isoform 1 localizes predominantly to the nucleus and Cytoplasm. Nucleus. Isoform 4 localizes predominantly to the cytoplasm. The cellular background in which it is expressed can influence whether it resides primarily in the cytoplasm or is also found in the nucleus. In the presence of BCL2, localizes to intracellular membranes (what appears to be the nuclear envelope and perinuclear membranes) as well as punctate cytosolic structures suggestive of mitochondria. - Information by UniProt
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参照データベース
- Entrez Gene: 573 Human
- Entrez Gene: 12017 Mouse
- Entrez Gene: 297994 Rat
- Omim: 601497 Human
- SwissProt: Q99933 Human
- SwissProt: Q60739 Mouse
- SwissProt: B0K019 Rat
- Unigene: 377484 Human
see all -
別名
- Bag 1 antibody
- BAG family molecular chaperone regulator 1 antibody
- BAG-1 antibody
see all
画像
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Anti-Bag1 antibody [Y166] (ab32109) at 1/500 dilution + HeLa cell lysate
Predicted band size: 31 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
Additional bands at: 46 kDa (possible isoform), 50 kDa (possible isoform) -
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using ab32109 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent staining of HeLa cells using ab32109 at 1/100 dilution.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab32109 は 9 報の論文で使用されています。
- Luo S et al. Bag-1 mediates glucocorticoid receptor trafficking to mitochondria after corticosterone stimulation: Potential role in regulating affective resilience. J Neurochem 158:358-372 (2021). PubMed: 33025573
- Lu S et al. Downregulation of BAG-1 in T47D cells promotes resistance to tamoxifen via activation of the PI3K/Akt/mTOR signaling pathway. Oncol Rep 41:1901-1910 (2019). PubMed: 30747221
- Gennaro VJ et al. Interaction between the BAG1S isoform and HSP70 mediates the stability of anti-apoptotic proteins and the survival of osteosarcoma cells expressing oncogenic MYC. BMC Cancer 19:258 (2019). PubMed: 30902071
- Miao JT et al. LncRNA ANRIL affects the sensitivity of ovarian cancer to cisplatin via regulation of let-7a/HMGA2 axis. Biosci Rep 39:N/A (2019). PubMed: 31189742
- Shen Z et al. miR-494-BAG-1 axis is involved in cinobufacini-induced cell proliferation and apoptosis in gastric cancer. Mol Med Rep 17:7435-7441 (2018). PubMed: 29568970
- Wood RJ et al. A biosensor-based framework to measure latent proteostasis capacity. Nat Commun 9:287 (2018). PubMed: 29348634
- Cato L et al. Development of Bag-1L as a therapeutic target in androgen receptor-dependent prostate cancer. Elife 6:N/A (2017). IHC-P, WB ; Human . PubMed: 28826504
- Mashukova A et al. The BAG-1 isoform BAG-1M regulates keratin-associated Hsp70 chaperoning of aPKC in intestinal cells during activation of inflammatory signaling. J Cell Sci 127:3568-77 (2014). PubMed: 24876225
- Anderson LR et al. BAG-1 overexpression attenuates luminal apoptosis in MCF-10A mammary epithelial cells through enhanced RAF-1 activation. Oncogene 29:527-38 (2010). ICC/IF ; Human . PubMed: 19881545