製品の概要

  • 製品名Anti-Bag1 antibody
    Bag1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Bag1
  • アプリケーション適用あり: WB, IHC-P, ICCmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide corresponding to a sequence at the C-terminal of Human Bag1.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab112493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. The detection limit for Bag1 is approximately 2.5ng/lane under non-reducing and reducing conditions.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli.
  • 組織特異性Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines (at protein level).
  • ��列類似性Contains 1 BAG domain.
    Contains 1 ubiquitin-like domain.
  • 翻訳後修飾Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
  • 細胞内局在Cytoplasm. Nucleus. Isoform 2 localizes to the cytoplasm and shuttles into the nucleus in response to heat shock; Nucleus. Cytoplasm. Isoform 1 localizes predominantly to the nucleus and Cytoplasm. Nucleus. Isoform 4 localizes predominantly to the cytoplasm. The cellular background in which it is expressed can influence whether it resides primarily in the cytoplasm or is also found in the nucleus. In the presence of BCL2, localizes to intracellular membranes (what appears to be the nuclear envelope and perinuclear membranes) as well as punctate cytosolic structures suggestive of mitochondria.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Bag 1 antibody
    • BAG family molecular chaperone regulator 1 antibody
    • BAG-1 antibody
    • BAG1 antibody
    • BAG1_HUMAN antibody
    • BCL 2 Associated Athanogene 1 antibody
    • BCL 2 associated athanogene antibody
    • BCL 2 binding athanogene 1 antibody
    • Bcl-2-associated athanogene 1 antibody
    • BCL2 associated athanogene 1 antibody
    • BCL2 associated athanogene antibody
    • Glucocorticoid receptor-associated protein RAP46 antibody
    • HAP1 antibody
    • Haptoglobin antibody
    • RAP 46 antibody
    • RAP46 antibody
    see all

Anti-Bag1 antibody 画像

  • All lanes : Anti-Bag1 antibody (ab112493)

    Lane 1 : Recombinant Human BAG-1 Protein at 0.01 µg
    Lane 2 : Recombinant Human BAG-1 Protein at 0.005 µg
    Lane 3 : Recombinant Human BAG-1 Protein at 0.0025 µg
    Lane 4 : MCF-7 Whole Cell Lysate


    Predicted band size : 39 kDa
    Observed band size : 39 kDa
  • ab112493 staining Bag1 in Rat intestine by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Detection by ABC/DAB staining.

Anti-Bag1 antibody (ab112493) 使用論文

ab112493 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"