The lyophilized antibody is stable for at least 2 years from date of receipt at -200C. The reconstituted antibody is stable for at least two weeks at 2-80C. Frozen aliquots are stable for at least 6 months when stored at -200C. Avoid repeated freeze-thaw cycles.
Lyophilised:Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 2 µg/ml.
Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Goat polyclonal to BAFF (Biotin) (ab83511). This antigen affinity purified antibody, in conjunction with ab83511 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant human BAFF.
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 31 kDa.
Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.
Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas.
Belongs to the tumor necrosis factor family.
The soluble form derives from the membrane form by proteolytic processing. N-glycosylated.