製品の概要

  • 製品名Anti-BAF57/SMARCE1 antibody
    BAF57/SMARCE1 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to BAF57/SMARCE1
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide:

    PPTDPIPEDEKKE

    , corresponding to amino acids 399-411 of Human BAF57/SMARCE1.

  • ポジティブ・コントロール
    • Lysates prepared from HepG2, HeLa and Jurkat cells. Lysate prepared from transfected HEK293 transiently expressing BAF57. Human prostate tissue sections.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab14764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).
IHC-P Use a concentration of 3 - 5 µg/ml.

ターゲット情報

  • 機能Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Required for the coactivation of estrogen responsive promoters by Swi/Snf complexes and the SRC/p160 family of histone acetyltransferases (HATs). Also specifically interacts with the CoREST corepressor resulting in repression of neuronal specific gene promoters in non-neuronal cells. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
  • 配列類似性Contains 1 HMG box DNA-binding domain.
  • ドメインThe HMG domain is essential for CD4 silencing and CD8 activation; mutation of this domain blocks thymus development.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BAF57 antibody
    • BRG1 associated factor 57 antibody
    • BRG1-associated factor 57 antibody
    • Chromatin remodeling complex BRG1 associated factor 57 antibody
    • FLJ35648 antibody
    • SMARCE 1 antibody
    • SMARCE1 antibody
    • SMCE1_HUMAN antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin e1 antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily e member 1 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1 antibody
    see all

Anti-BAF57/SMARCE1 antibody 画像

Anti-BAF57/SMARCE1 antibody (ab14764) 使用論文

This product has been referenced in:
  • Miyamoto K  et al. Nuclear actin polymerization is required for transcriptional reprogramming of Oct4 by oocytes. Genes Dev 25:946-58 (2011). ChIP, IP ; Xenopus laevis . Read more (PubMed: 21536734) »
  • Kouzu-Fujita M  et al. Coactivation of estrogen receptor beta by gonadotropin-induced cofactor GIOT-4. Mol Cell Biol 29:83-92 (2009). WB ; Human . Read more (PubMed: 18981223) »

See all 2 Publications for this product

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