製品の概要
- 製品名Anti-BAF53A antibody
BAF53A 一次抗体 製品一覧 - 製品の詳細Rabbit polyclonal to BAF53A
- アプリケーション適用あり: IHC-P, ICC/IFmore details
- 種交差性交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
- 免疫原
Synthetic peptide, corresponding to amino acids 25-75 of human BRG1-associated factor, 53 kDa subunit A (NP_004292.1)
- ポジティブ･コントロール
- Human controls: Breast Carcinoma, Colon Carcinoma, Linitis Plastica Stomach Cancer, Ovarian Carcinoma, Pancreatic Islet
Cell Tumor, Prostate Carcinoma, Skin Squamous Cell Carcinoma, Stomach Adenocarcinoma.
Mouse controls: Colon Carcinoma CT26, Teratoma
- Human controls: Breast Carcinoma, Colon Carcinoma, Linitis Plastica Stomach Cancer, Ovarian Carcinoma, Pancreatic Islet
Cell Tumor, Prostate Carcinoma, Skin Squamous Cell Carcinoma, Stomach Adenocarcinoma.
製品の特性
- 製品の状態Liquid
- 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- バッファーPreservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... - 精製度Immunogen affinity purified
- 特記事項（精製）Antibody was affinity purified using an epitope specific to BAF53A immobilized on solid support.
- ポリ/モノポリクローナル
- アイソタイプIgG
- 研究分野
関連製品
- Compatible Secondaries
- Positive Controls
- Breast tumor (human): adenocarcinoma (mucous) tissue slides (ab4701)
- Colon tumor (human): adenocarcinoma (mucoid) tissue slides (ab4703)
- Pancreas tumor (human): islet cell tumor tissue slides (ab5038)
- Prostate tumor (human): adenocarcinoma tissue slides (ab5051)
- Skin tumor (human): squamous cell carcinoma (well differentiated) tissue slides (ab5107)
アプリケーション
Our Abpromise guarantee covers the use of ab84486 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | 1/100 - 1/500. Antigen retrieval is not essential but may optimise staining. The use of citrate buffer will enhance staining. | |
| ICC/IF | Use at an assay dependent concentration. |
ターゲット情報
- 関連性This protein is a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This protein is a 53 kDa subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. It is required for maximal ATPase activity of SMARCA4/BRG1 and for association of the SMARCA4/BRG1 containing remodelling complex BAF with chromatin/nuclear matrix. It is a component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage.
- 細胞内局在Nuclear
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参照データベース
- Entrez Gene: 100125416 Cow
- Entrez Gene: 86 Human
- Entrez Gene: 56456 Mouse
- Entrez Gene: 361925 Rat
- Omim: 604958 Human
- SwissProt: A4FUX8 Cow
- SwissProt: O96019 Human
- SwissProt: Q9Z2N8 Mouse
see all -
別名
- 53 kDa BRG1 associated factor A antibody
- Actin like 6A antibody
- Actin like protein 6A antibody
see all
Anti-BAF53A antibody 画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BAF53A antibody (ab84486)ab84486 at 1/250 dilution staining formalin-fixed, paraffin-embedded section of human breast carcinoma by immunohistochemistry.
Detection: DAB staining. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BAF53A antibody (ab84486)ab84486 at 1/250 dilution staining formalin-fixed, paraffin-embedded section of mouse teratoma by immunohistochemistry.
Detection: DAB staining. -
Immunocytochemistry/ Immunofluorescence - BAF53A antibody (ab84486)ab84486 used a dilution of 1/100 staining formalin-fixed, paraffin-embedded section of human breast carcinoma by immunofluorescence.
Detection: Red-fluorescent goat anti-rabbit IgG.
Anti-BAF53A antibody (ab84486) 使用論文
ab84486 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"