Anti-Bad (phospho S155) 抗体 (ab28825)
Key features and details
- Rabbit polyclonal to Bad (phospho S155)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Bad (phospho S155) antibody
Bad 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Bad (phospho S155) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Bad (phospho S155).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab28825の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 18 kDa).
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IHC-P |
Use at an assay dependent concentration.
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 18 kDa). |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways. -
組織特異性
Expressed in a wide variety of tissues. -
配列類似性
Belongs to the Bcl-2 family. -
ドメイン
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. -
翻訳後修飾
Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2. -
細胞内局在
Mitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 572 Human
- Omim: 603167 Human
- SwissProt: Q92934 Human
- Unigene: 370254 Human
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別名
- AI325008 antibody
- BAD antibody
- BAD_HUMAN antibody
see all
画像
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Ab28825, at a concentration of 1/50, staining Bad in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
Left image: untreated.
Right image: treated with synthesized phosphopeptide. -
Anti-Bad (phospho S155) antibody (ab28825) at 1/500 dilution + extracts from 293 cells (5-30ug) treated with forsklin
Predicted band size: 18 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab28825 は 5 報の論文で使用されています。
- Boac BM et al. Expression of the BAD pathway is a marker of triple-negative status and poor outcome. Sci Rep 9:17496 (2019). PubMed: 31767884
- Ke J et al. Edaravone reduces oxidative stress and intestinal cell apoptosis after burn through up-regulating miR-320 expression. Mol Med 25:54 (2019). PubMed: 31829167
- Zhao L et al. Protein kinase A determines platelet life span and survival by regulating apoptosis. J Clin Invest 127:4338-4351 (2017). PubMed: 29083324
- Rong F et al. Knockdown of RhoGDIα induces apoptosis and increases lung cancer cell chemosensitivity to paclitaxel. Neoplasma 59:541-50 (2012). PubMed: 22668020
- Danial NN et al. Dual role of proapoptotic BAD in insulin secretion and beta cell survival. Nat Med 14:144-53 (2008). PubMed: 18223655