製品の概要

  • 製品名Anti-Bad (phospho S134) antibody
    Bad 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Bad (phospho S134)
  • 特異性ab62212 detects endogenous levels of Bad only when phosphorylated at serine 134 (Human: Ser134; Mouse: Ser170; Rat: Ser171).
  • アプリケーション適用あり: IHC-P, ICC/IF, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic phosphopeptide derived from human Bad around the phosphorylation site of serine 134 (P-K-SP-A-G).

  • ポジティブ・コントロール
    • Human brain tissue. HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab62212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.

ターゲット情報

  • 機能Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
  • 組織特異性Expressed in a wide variety of tissues.
  • 配列類似性Belongs to the Bcl-2 family.
  • ドメインIntact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • 翻訳後修飾Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2.
  • 細胞内局在Mitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AI325008 antibody
    • BAD antibody
    • BAD_HUMAN antibody
    • BBC 2 antibody
    • BBC2 antibody
    • BBC6 antibody
    • Bcl 2 Antagonist of Cell Death antibody
    • Bcl 2 Binding Component 6 antibody
    • BCL X / BCL 2 Binding Protein antibody
    • BCL X Binding Protein antibody
    • Bcl XL/Bcl 2 Associated Death Promoter antibody
    • Bcl-2-binding component 6 antibody
    • Bcl-2-like protein 8 antibody
    • Bcl-XL/Bcl-2-associated death promoter antibody
    • Bcl2 antagonist of cell death antibody
    • BCL2 antagonist of cell death protein antibody
    • BCL2 associated agonist of cell death antibody
    • Bcl2 Associated Death Promoter antibody
    • BCL2 binding component 6 antibody
    • BCL2 binding protein antibody
    • Bcl2 Like 8 Protein antibody
    • Bcl2-L-8 antibody
    • BCL2L8 antibody
    • Proapoptotic BH3 Only Protein antibody
    see all

Anti-Bad (phospho S134) antibody 画像

  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using Bad (phospho S134) antibody (ab62212) at 1/50 dilution, in the presence (right) and absence (left) of immunising phosphopeptide.
  • Immunofluorescence analysis of HeLa cells using Bad (phospho S134) antibody (ab62212) at 1/500 dilution, in the presence (right) and absence (left) of immunising phosphopeptide.

Anti-Bad (phospho S134) antibody (ab62212) 使用論文

ab62212 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"