製品の概要

  • 製品名Anti-Bad antibody
    Bad 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Bad
  • アプリケーション適用あり: ICC/IF, IHC-Fr, IHC-P, IP, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length Human Bad protein (NP_004313).

  • ポジティブ・コントロール
    • Spleen, lymphoid, Du145 Human prostate carcinoma cells, many cancer cell lines.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab97733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/500 - 1/2000.
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/1000 - 1/5000.
IP 1/50 - 1/200.
WB 1/1000 - 1/2000. Predicted molecular weight: 18 kDa.

ターゲット情報

  • 機能Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
  • 組織特異性Expressed in a wide variety of tissues.
  • 配列類似性Belongs to the Bcl-2 family.
  • ドメインIntact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • 翻訳後修飾Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2.
  • 細胞内局在Mitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AI325008 antibody
    • BAD antibody
    • BAD_HUMAN antibody
    • BBC 2 antibody
    • BBC2 antibody
    • BBC6 antibody
    • Bcl 2 Antagonist of Cell Death antibody
    • Bcl 2 Binding Component 6 antibody
    • BCL X / BCL 2 Binding Protein antibody
    • BCL X Binding Protein antibody
    • Bcl XL/Bcl 2 Associated Death Promoter antibody
    • Bcl-2-binding component 6 antibody
    • Bcl-2-like protein 8 antibody
    • Bcl-XL/Bcl-2-associated death promoter antibody
    • Bcl2 antagonist of cell death antibody
    • BCL2 antagonist of cell death protein antibody
    • BCL2 associated agonist of cell death antibody
    • Bcl2 Associated Death Promoter antibody
    • BCL2 binding component 6 antibody
    • BCL2 binding protein antibody
    • Bcl2 Like 8 Protein antibody
    • Bcl2-L-8 antibody
    • BCL2L8 antibody
    • Proapoptotic BH3 Only Protein antibody
    see all

Anti-Bad antibody 画像

  • Immunofluorescence analysis of Bad expression using ab97733 at 1/2000, followed by a FITC-conjugated secondary antibody..
    Du145 Human prostate carcinoma cells were cultured without (A) or with (B) 1µM of the triphosphatase inhibitor thapsigargin (THG) for 12 hr.

    THG induces Ca2+ release from internal stores which can promote apoptosis. BAD staining was located diffusely throughout the cytoplasm of untreated cells (A), and localized to the mitochondria in treated cells (B).

Anti-Bad antibody (ab97733) 使用論文

ab97733 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"