製品の概要

  • 製品名Anti-Bad antibody [64103]
    Bad 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [64103] to Bad
  • 特異性This antibody will also detect a 32 kDa band that comigrates with recombinant Bad on immunoblots.
  • アプリケーション適用あり: Dot blotmore details
  • 種交差性
    交差種: Mouse
    非交差種: Human
  • 免疫原

    Recombinant N-Termanal fragment (Mouse).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab10520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Dot blot
  • 追加情報Dot: a working antibody concentration of approximately 1 µg/ml is recommended using extracts from 2 x 106 mouse cytotoxic T cell line (CTLL2) or L929 fibroblast cells in an enhanced chemiluminescent (ECL) assay.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
    • 組織特異性Expressed in a wide variety of tissues.
    • 配列類似性Belongs to the Bcl-2 family.
    • ドメインIntact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
    • 翻訳後修飾Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2.
    • 細胞内局在Mitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AI325008 antibody
      • BAD antibody
      • BAD_HUMAN antibody
      • BBC 2 antibody
      • BBC2 antibody
      • BBC6 antibody
      • Bcl 2 Antagonist of Cell Death antibody
      • Bcl 2 Binding Component 6 antibody
      • BCL X / BCL 2 Binding Protein antibody
      • BCL X Binding Protein antibody
      • Bcl XL/Bcl 2 Associated Death Promoter antibody
      • Bcl-2-binding component 6 antibody
      • Bcl-2-like protein 8 antibody
      • Bcl-XL/Bcl-2-associated death promoter antibody
      • Bcl2 antagonist of cell death antibody
      • BCL2 antagonist of cell death protein antibody
      • BCL2 associated agonist of cell death antibody
      • Bcl2 Associated Death Promoter antibody
      • BCL2 binding component 6 antibody
      • BCL2 binding protein antibody
      • Bcl2 Like 8 Protein antibody
      • Bcl2-L-8 antibody
      • BCL2L8 antibody
      • Proapoptotic BH3 Only Protein antibody
      see all

    Anti-Bad antibody [64103] (ab10520) 使用論文

    ab10520 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab10520.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"