The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報ELISA: Use at an assay dependent dilution. Can be used in a sandwich ELISA along with ab1994.
WB: Use at a concentration of 2 µg/ml. (Under non-reducing conditions) Detects a band of approximately 92-94 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
関連性Bacillus anthracis is an aerobic spore forming bacterium that causes anthrax, an acute infectious disease of cattle, sheep, goats, camels, antelopes and other herbivores. Infection can occur in humans when they are exposed to infected animals or tissue from infected animals via three routes: cutaneous, inhalation and gastrointestinal. B. anthracis spores persist in the soil for many years as they are resistant to heat, cold, radiation, desiccation, and disinfectants. In vivo, B anthracis produces a polypeptide (polyglutamic acid) capsule that protects it from phagocytosis. The virulence factors of B anthracis are its capsule and three component toxin, both encoded on plasmids.
Western blot - Bacillus anthracis spore antigen antibody [SA26] (ab2281)
ab2281 at 2mg/ml staining Bacillus anthracis spore antigen.
Lane 1 - clone SA26
Lane 2 - clone SA27
Lane 3 - monoclonal antibody against protective antigen of Bacillus Anthracis -C3 (ab8240)
Lane 4 - monoclonal antibody against protective antigen of Bacillus Anthracis -BAP0104 (ab1991)
Lane 5 - control a-IgG(mouse) - HRP
Lane 6 - rabbit polyclonal antibody against Bacillus Anthracis Spore antigen (ab8244).
Lane 7 - rabbit polyclonal antibody against Bacillus Anthracis Spore antigen (ab8244)
Lane 8 - control a-IgG (rabbit) - HRP
Lane 9 - B.anthracis spores
Lane 10 - Molecular mass standards from top to bottom 94K, 67K, 43K, 30K, 20K; Amidoblack stain.
After washing, the nitrocellulose was incubated with anti-mouse IgG - HRP or anti-rabbit IgG-HRP conjugates at working dilutions 1:25000 and 1:500 respectively. Later on the substrate solution, containing diaminobenzidine with 4-chloro-1-naphtol was added.
Sanz P et al. Detection of Bacillus anthracis spore germination in vivo by bioluminescence imaging. Infect Immun76:1036-47 (2008).
Read more (PubMed: 18195028) »
Williams DD & Turnbough CL Surface layer protein EA1 is not a component of Bacillus anthracis spores but is a persistent contaminant in spore preparations. J Bacteriol186:566-9 (2004).
Read more (PubMed: 14702325) »