Anti-Bacillus anthracis lethal factor 抗体 [BAL0106] (ab8243)


  • 製品名
    Anti-Bacillus anthracis lethal factor antibody [BAL0106]
    Bacillus anthracis lethal factor 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [BAL0106] to Bacillus anthracis lethal factor
  • アプリケーション
    適用あり: ELISA, Sandwich ELISAmore details
  • 免疫原

    Highly purified lethal factor antigen of Bacillus anthracis.

  • 特記事項

    Detection of lethal factor of B. anthracis

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.


  • 製品の状態
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    PBS with 0.1% sodium azide
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    Purified by chromatography on protein G Sepharose. Purity is tested by electrophoresis.
  • 一次抗体 備考
    Detection of lethal factor of B. anthracis
  • ポリ/モノ
  • クローン名
  • ミエローマ
  • アイソタイプ
  • 研究分野


Our Abpromise guarantee covers the use of ab8243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent dilution. Can be paired for ELISA with Mouse monoclonal [BAL0105] to Bacillus anthracis lethal factor (HRP) (ab24451). Can be used as Detection antibody.
Sandwich ELISA Use at an assay dependent dilution.


  • 関連性
    The protease enzyme Lethal Factor (LF) is one of the three proteins (LF, EF & PA) composing the anthrax toxin produced by Bacillus anthracis, a bacteria which can infect many mammalian species and that may be fatal. LF is not toxic by itself, but when associated with Protective Antigen (PA), can then gain entry to cells. Once inside the cell, LF then cleaves the N terminal of most dual specificity mitogen activated protein kinase kinases (MAPKKs or MAP2Ks) (except for MAP2K5). Cleavage invariably occurs within the N terminal proline rich region preceding the kinase domain, thus disrupting a sequence involved in directing specific protein protein interactions necessary for the assembly of signaling complexes. There may be other cytosolic targets of LF involved in cytotoxicity. The proteasome may mediate a toxic process initiated by LF in the cell cytosol involving degradation of unidentified molecules that are essential for macrophage homeostasis. This is an early step in LF intoxication, but it is downstream of the cleavage by LF of MEK1 or other putative substrates.
  • 細胞内局在
  • 別名
    • Anthrax lethal toxin endopeptidase component antibody
    • Anthrax LF antibody
    • Lef antibody
    • Lethal factor antibody
    • LF antibody
    see all


  • Calibration curve in ELISA for B.anthracis Lethal Factor Antigen determination using ab8242 for coating and ab8243 as a HRP conjugate.



ab8243 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for contacting us.
When ab8243 is stored properly aliquoted and at -20C, it should be viable for up to 5 years.
When ab102891 is stored properly at -20C, the kit should be viable for a year. Once the labeling is done with the kit, t...

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Thank you for contacting us.

I have had the oportunity to look over the data regarding the antibodies we had talked about this morning. I am confident that your assessment of those products we correct as ab8242 and ab24451 have been created ...

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Thank you for your enquiry. Both ab8243 and ab24451 were generated using highly purified lethal factor antigen of Bacillus anthracis. These two antibodies can be used together in a sandwich ELISA where ab8243 is the coating antibody and ab24451 is the ...

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Thank you for your enquiry. Ab8243 is not HRP conjugated. You may use Ab24451 for detection in place of this antibody, as it is HRP conjugated. I hope this information helps, please do not hesitate to contact us if you need any more advice or in...

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Thank you for your enquiry. Our source has replied: "The normal presentation of the antibodies is non-conjugated, but we are also offering them in conjugated form. We are normally not using anti-mouse IgG secondary antibodies, we are using hrp-conjug...

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