Anti-Bacillus anthracis lethal factor 抗体 (ab13805)

製品の概要

  • 製品名Anti-Bacillus anthracis lethal factor antibody
    Bacillus anthracis lethal factor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Bacillus anthracis lethal factor
  • 特異性The antibody has been tested against recombinant protein only (0.1 ug recombinant protein was loaded per lane).
  • アプリケーション適用あり: WBmore details
  • 種交差性
    Anthrax LF (Lethal Factor) produced by Bacillus anthracis
  • 免疫原

    Synthetic peptide, corresponding to amino acids 47-65 of Anthrax LF

  • ポジティブ・コントロール
    • 0.1 ug recombinant protein

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.05% Sodium Azide
    Constituents: 0.2% gelatin, PBS
  • 精製度Whole antiserum
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab13805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 90 kDa.

ターゲット情報

  • 関連性The protease enzyme Lethal Factor (LF) is one of the three proteins (LF, EF & PA) composing the anthrax toxin produced by Bacillus anthracis, a bacteria which can infect many mammalian species and that may be fatal. LF is not toxic by itself, but when associated with Protective Antigen (PA), can then gain entry to cells. Once inside the cell, LF then cleaves the N terminal of most dual specificity mitogen activated protein kinase kinases (MAPKKs or MAP2Ks) (except for MAP2K5). Cleavage invariably occurs within the N terminal proline rich region preceding the kinase domain, thus disrupting a sequence involved in directing specific protein protein interactions necessary for the assembly of signaling complexes. There may be other cytosolic targets of LF involved in cytotoxicity. The proteasome may mediate a toxic process initiated by LF in the cell cytosol involving degradation of unidentified molecules that are essential for macrophage homeostasis. This is an early step in LF intoxication, but it is downstream of the cleavage by LF of MEK1 or other putative substrates.
  • 細胞内局在secreted
  • 別名
    • Anthrax lethal toxin endopeptidase component antibody
    • Anthrax LF antibody
    • Lef antibody
    • Lethal factor antibody
    • LF antibody
    see all

Anti-Bacillus anthracis lethal factor antibody 画像

  • Western blot analysis of Anthrax LF in recombinant protein using ab13805 (lane B) at 1:1000 dilution. Lane A shows the pre-bleed.

Anti-Bacillus anthracis lethal factor antibody (ab13805) 使用論文

ab13805 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. The originator of ab13805 has informed us that the purity of the antibody is whole antiserum and since it is unpurified antibody, a concentration cannot be determined. Please contact us again if you have any additional qu...

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Thank you for your enquiry. Both of these antibodies are sold as sera and therefore concentration of specific antibody has not been determined. Recommended dilutions are given on the online datasheets, and I would recommend referring to those. If you h...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"