製品の概要

  • 製品名Anti-BACE2 antibody
    BACE2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to BACE2
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 496-511 of Human BACE 2.

  • ポジティブ・コントロール
    • Human heart tissue lysate. This antibody gave a positive result when used in the following methanol fixed cell lines: MALME-3M
  • 特記事項Beta-site APP Cleaving Enzyme

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab8024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 2 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Can be blocked with BACE2 peptide (ab8391).

ターゲット情報

  • 機能Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672.
  • 組織特異性Brain. Present in neurons within the hippocampus, frontal cortex and temporal cortex (at protein level). Expressed at low levels in most peripheral tissues and at higher levels in colon, kidney, pancreas, placenta, prostate, stomach and trachea. Expressed at low levels in the brain. Found in spinal cord, medulla oblongata, substantia nigra and locus coruleus. Expressed in the ductal epithelium of both normal and malignant prostate.
  • 配列類似性Belongs to the peptidase A1 family.
  • 翻訳後修飾Undergoes autoproteolytic cleavage.
    Glycosylated.
  • 細胞内局在Membrane. Golgi apparatus. Endoplasmic reticulum. Endosome. Cell surface.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ALP 56 antibody
    • ALP56 antibody
    • Asp 1 antibody
    • ASP1 antibody
    • ASP21 antibody
    • Aspartic Like Protease 56kDa antibody
    • Aspartic-like protease 56 kDa antibody
    • Aspartyl protease 1 antibody
    • BACE 2 antibody
    • Bace2 antibody
    • BACE2_HUMAN antibody
    • Beta Secretase 2 antibody
    • Beta Site Amyloid Beta A4 Precursor Protein Cleaving Enzyme 2 antibody
    • Beta-secretase 2 antibody
    • Beta-site amyloid precursor protein cleaving enzyme 2 antibody
    • Beta-site APP cleaving enzyme 2 antibody
    • Down region aspartic protease antibody
    • Downs Syndrome Region Aspartic Protease antibody
    • DRAP antibody
    • Memapsin 1 antibody
    • Memapsin-1 antibody
    • Membrane associated aspartic protease 1 antibody
    • Membrane-associated aspartic protease 1 antibody
    • Theta-secretase antibody
    see all

Anti-BACE2 antibody 画像

  • ICC/IF image of ab8024 stained MALME-3M cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab8024 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-BACE2 antibody (ab8024) at 1 µg/ml

    Lane 1 : human heart tissue lysate with absence of blocking peptide
    Lane 2 : human heart tissue lysate with presence of blocking peptide
  • Immunohistochemistry of BACE2 in rat heart tissue with BACE2 antibody at 2 µg/ml.

Anti-BACE2 antibody (ab8024) 使用論文

ab8024 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"