Anti-BACE2 抗体 (ab5670)
Key features and details
- Rabbit polyclonal to BACE2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-BACE2 antibody
BACE2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to BACE2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human BACE2 aa 441-457.
Sequence:VSEISGPFSTEDIASNC
(Peptide available asab5869) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5670の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 2 µg/ml.
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ICC/IF |
1/10 - 1/200.
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特記事項 |
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WB
Use a concentration of 2 µg/ml. |
ICC/IF
1/10 - 1/200. |
ターゲット情報
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機能
Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672. -
組織特異性
Brain. Present in neurons within the hippocampus, frontal cortex and temporal cortex (at protein level). Expressed at low levels in most peripheral tissues and at higher levels in colon, kidney, pancreas, placenta, prostate, stomach and trachea. Expressed at low levels in the brain. Found in spinal cord, medulla oblongata, substantia nigra and locus coruleus. Expressed in the ductal epithelium of both normal and malignant prostate. -
配列類似性
Belongs to the peptidase A1 family. -
翻訳後修飾
Undergoes autoproteolytic cleavage.
Glycosylated. -
細胞内局在
Membrane. Golgi apparatus. Endoplasmic reticulum. Endosome. Cell surface. - Information by UniProt
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参照データベース
- Entrez Gene: 25825 Human
- Entrez Gene: 56175 Mouse
- Omim: 605668 Human
- SwissProt: Q9Y5Z0 Human
- SwissProt: Q9JL18 Mouse
- Unigene: 529408 Human
- Unigene: 97885 Mouse
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別名
- ALP 56 antibody
- ALP56 antibody
- Asp 1 antibody
see all
画像
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Immunocytochemistry/Immunofluorescence analysis of BACE2 (green) showing staining in the nucleus and cytoplasm of A549 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5670 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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All lanes : Anti-BACE2 antibody (ab5670) at 1/500 dilution
Lane 1 : Mouse spleen cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : HL60 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 25 µg per lane.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (4)
ab5670 は 4 報の論文で使用されています。
- Chen Z et al. Cell surface GRP78 regulates BACE2 via lysosome-dependent manner to maintain mesenchymal phenotype of glioma stem cells. J Exp Clin Cancer Res 40:20 (2021). PubMed: 33413577
- Cao K et al. Exposure to fluoride aggravates the impairment in learning and memory and neuropathological lesions in mice carrying the APP/PS1 double-transgenic mutation. Alzheimers Res Ther 11:35 (2019). PubMed: 31010414
- Holler CJ et al. BACE2 expression increases in human neurodegenerative disease. Am J Pathol 180:337-50 (2012). PubMed: 22074738
- Ahmed RR et al. BACE1 and BACE2 enzymatic activities in Alzheimer's disease. J Neurochem 112:1045-53 (2010). WB ; Human . PubMed: 19968762