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Synthetic peptide, corresponding to amino acids 485-501 of Human BACE. The peptide sequence differs fromthose of mouse and rat BACE by one amino acid (1).
Beta-site APP Cleaving Enzyme.
Our Abpromise guarantee covers the use of ab2077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use a concentration of 10 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa.Can be blocked with BACE1 peptide (ab7883). We recommend overnight incubation at 4C and using 5% skim milk to block.|
|ICC/IF||Use at an assay dependent concentration.|
Immunocytochemical analysis of NIH/3T3 cells, labeling BACE1 with ab2077 (20µg/ml).
Immunohistochemistry of BACE in mouse brain tissue with BACE antibody at 2.5 µg/ml.