製品の概要

  • 製品名Anti-BACE1 antibody
    BACE1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to BACE1
  • 特異性Detects transfected beta-secretase 1.
  • アプリケーション適用あり: IP, ELISA, ICC, ICC/IF, IHC-FoFr, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Guinea pig, Cow
  • 免疫原

    Synthetic peptide corresponding to Human BACE1 aa 485-501 (C terminal).
    Sequence:

    CLRQQHDDFADDISLLK


    (Peptide available as ab7883)

  • ポジティブ・コントロール
    • HeLa cells transfected with the human gene.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab10716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC 1/20 - 1/200.
ICC/IF 1/20 - 1/200.
IHC-FoFr Use at an assay dependent concentration.
WB 1/200 - 1/2000. Detects a band of approximately 70 kDa (predicted molecular weight: 60 kDa).Can be blocked with BACE1 peptide (ab7883).

Detects a band of approximately 70 kDa from HeLa cells transfected with the human gene.

ターゲット情報

  • 機能Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase.
  • 組織特異性Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.
  • 配列類似性Belongs to the peptidase A1 family.
  • ドメインThe transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP.
  • 翻訳後修飾Glycosylated.
  • 細胞内局在Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface.
  • Information by UniProt
  • 参照データベース
  • 別名
    • APP beta secretase antibody
    • Asp 2 antibody
    • ASP2 antibody
    • Aspartyl protease 2 antibody
    • BACE 1 antibody
    • BACE antibody
    • BACE1 antibody
    • BACE1_HUMAN antibody
    • Beta secretase 1 antibody
    • Beta secretase antibody
    • Beta site amyloid beta A4 precursor protein cleaving enzyme antibody
    • Beta site amyloid precursor protein cleaving enzyme 1 antibody
    • Beta site amyloid precursor protein cleaving enzyme antibody
    • Beta site APP cleaving enzyme 1 antibody
    • Beta site APP cleaving enzyme antibody
    • Beta-secretase 1 antibody
    • Beta-site amyloid precursor protein cleaving enzyme 1 antibody
    • Beta-site APP cleaving enzyme 1 antibody
    • FLJ90568 antibody
    • HSPC104 antibody
    • Memapsin 2 antibody
    • Memapsin-2 antibody
    • Memapsin2 antibody
    • Membrane associated aspartic protease 2 antibody
    • Membrane-associated aspartic protease 2 antibody
    • Transmembrane aspartic proteinase Asp2 antibody
    see all

Anti-BACE1 antibody 画像

  • Immunocytochemistry/Immunofluorescent analysis of BACE1 (green) showing staining in the Golgi apparatus of SH-SY5Y cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a10716 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 �C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunocytochemistry/Immunofluorescent analysis of BACE1 (green) showing staining in the Golgi apparatus of Neuro-2a cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab10716 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 �C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunocytochemistry/Immunofluorescent analysis of BACE1 (green) showing staining in the Golgi apparatus of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab10716 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 �C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Anti-BACE1 antibody (ab10716) 使用論文

ab10716 has not yet been referenced specifically in any publications.

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