製品の概要

  • 製品名
    Anti-B9D2 antibody
  • 製品の詳細
    Rabbit polyclonal to B9D2
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 30-60 of Human B9D2.

  • ポジティブ・コントロール
    • Mouse spleen tissue lysate; Human lung carcinoma tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab103961 is purified through a protein A column, followed by peptide affinity purification.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab103961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 19 kDa.

ターゲット情報

  • 関連性
    B9D2 is a B9 domain protein, which are exclusively found in ciliated organisms. The B9D2 gene is upregulated during mucociliary differentiation, and the encoded protein localizes to basal bodies and cilia. Disrupting expression of this gene results in ciliogenesis defects.
  • 細胞内局在
    Cytoplasm; cytoskeleton; cilium basal body; cilium axoneme. Nucleus.
  • 参照データベース
  • 別名
    • B9 domain containing protein 2 antibody
    • B9 protein domain 2 antibody
    • ICIS 1 antibody
    • Involved in cIlia stability 1 antibody
    • MGC4093 antibody
    • MGC41256 antibody
    • MKS1 related protein 2 antibody
    • MKS10 antibody
    • MKSR2 antibody
    • RGD1566122 antibody
    • Stumpy antibody
    • Stumpy, mouse, homolog of antibody
    see all

画像

  • Anti-B9D2 antibody (ab103961) at 1/100 dilution + Mouse spleen tissue lysate at 35 µg

    Predicted band size : 19 kDa
  • ab103961, at 1/50 dilution, staining B9D2 in formalin-fixed, paraffin-embedded Human lung carcinoma by Immunohistochemistry, using a peroxidase-conjugated secondary antibody and DAB staining.

参考文献

ab103961 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Mouse Tissue sections (Testes)
Specification
Testes
Permeabilization
Yes - Triton
Fixative
Acetone
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投稿 Nov 12 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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