製品の概要

  • 製品名Anti-B4GALT1 antibody
    B4GALT1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to B4GALT1
  • アプリケーション適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen, corresponding to amino acids 50-163 of Human B4GALT1 (P15291).

  • ポジティブ・コントロール
    • Human prostate tissue; U-251 MG cell lysate; A-431 cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab121326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.
    The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix.
  • 組織特異性Ubiquitously expressed, but at very low levels in fetal and adult brain.
  • パスウェイProtein modification; protein glycosylation.
  • 関連疾患Defects in B4GALT1 are the cause of congenital disorder of glycosylation type 2D (CDG2D) [MIM:607091]. CDGs are a family of severe inherited diseases caused by a defect in protein N-glycosylation. They are characterized by under-glycosylated serum proteins. These multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions.
  • 配列類似性Belongs to the glycosyltransferase 7 family.
  • 翻訳後修飾The soluble form derives from the membrane forms by proteolytic processing.
  • 細胞内局在Golgi apparatus > Golgi stack membrane. Cell membrane. Cell surface. Found in trans cisternae of Golgi; Golgi apparatus > Golgi stack membrane. Found in trans cisternae of Golgi and Secreted. Soluble form found in body fluids.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 384.Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase antibody
    • 4-galactosyltransferase 1 antibody
    • 4-GalTase 1 antibody
    • b4Gal-T1 antibody
    • B4galt1 antibody
    • B4GT1_HUMAN antibody
    • Beta-1 antibody
    • Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase antibody
    • Beta4Gal-T1 antibody
    • CDG2D antibody
    • GGTB2 antibody
    • GT1 antibody
    • GTB antibody
    • Lactose synthase A protein antibody
    • N-acetyllactosamine synthase antibody
    • Nal synthase antibody
    • Processed beta-1 antibody
    • UDP-Gal:beta-GlcNAc beta-1 antibody
    • UDP-galactose:beta-N-acetylglucosamine beta-1 antibody
    see all

Anti-B4GALT1 antibody 画像

  • All lanes : Anti-B4GALT1 antibody (ab121326) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human Plasma
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique
  • ab121326, at 1/125, staining B4GALT1 in paraffin embedded Human prostate tissue by Immunohistochemistry.
  • ab121326, at 4 µg/ml, staining B4GALT1 (green) in A-431 cells by Immunofluorescence.

Anti-B4GALT1 antibody (ab121326) 使用論文

ab121326 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample Human Cell (A549)
Specification A549
Permeabilization No
Fixative Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

投稿 Sep 30 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"