製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to B4GALNT3
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rabbit, Horse, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 252-301 (NEEGTDHVEV AWRRNDPGAK FTIIDSLSLS LFTNETFLQM DEVGHIPQTA) of Human B4GALNT3 (NP_775864).

  • ポジティブ・コントロール
    • HeLa cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab82800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

ターゲット情報

  • 機能
    Transfers N-acetylgalactosamine (GalNAc) from UDP-GalNAc to N-acetylglucosamine-beta-benzyl with a beta-1,4-linkage to form N,N'-diacetyllactosediamine, GalNAc-beta-1,4-GlcNAc structures in N-linked glycans and probably O-linked glycans. Mediates the N,N'-diacetyllactosediamine formation on gastric mucosa.
  • 組織特異性
    Highly expressed in testis, colon and stomach. Weakly expressed in other tissues.
  • 配列類似性
    Belongs to the chondroitin N-acetylgalactosaminyltransferase family.
  • 細胞内局在
    Golgi apparatus > Golgi stack membrane. Localizes to apical Golgi.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 4-N-acetylgalactosaminyltransferase 3 antibody
    • 4-N-acetylgalactosaminyltransferase III antibody
    • B4GalNAcT3 antibody
    • B4GALNT3 antibody
    • B4GN3_HUMAN antibody
    • Beta 1,4 N acetyl galactosaminyl transferase 3 antibody
    • Beta 1,4 N acetylgalactosaminyltransferase III antibody
    • Beta-1 antibody
    • Beta4GalNAc T3 antibody
    • Beta4GalNAc-T3 antibody
    • Beta4GalNAcT3 antibody
    • FLJ16224 antibody
    • FLJ40362 antibody
    • N acetyl beta glucosaminyl glycoprotein 4 beta N acetylgalactosaminyltransferase 2 antibody
    • N acetyl beta glucosaminyl glycoprotein 4 beta N acetylgalactosaminyltransferase antibody
    • N-acetyl-beta-glucosaminyl-glycoprotein 4-beta-N-acetylgalactosaminyltransferase 2 antibody
    • NGalNAc T2 antibody
    • NGalNAc-T2 antibody
    • NGalNAcT2 antibody
    • OTTHUMP00000159611 antibody
    see all

画像

  • Anti-B4GALNT3 antibody (ab82800) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 115 kDa
    Observed band size : 115 kDa

プロトコール

参考文献

This product has been referenced in:
  • Hirakawa T  et al. Wisteria floribunda agglutinin-binding glycan expression is decreased in endometriomata. Reprod Biol Endocrinol 12:100 (2014). WB ; Human . Read more (PubMed: 25344456) »

See 1 Publication for this product

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