製品の概要

  • 製品名Anti-B Raf antibody [EP152Y]
    B Raf 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP152Y] to B Raf
  • アプリケーション適用あり: WB, IHC-P, Flow Cyt, IPmore details
    適用なし: ICC
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide. within Human B Raf aa 50-150. The exact sequence is proprietary.
    Database link: P15056

  • ポジティブ・コントロール
    • HeLa cell lysate, human prostate cancer tissue. This antibody also reacts with rat brain tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody. Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available: 
    Anti-B Raf antibody (Phycoerythrin) [EP152Y] (ab210411)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab33899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000. Detects a band of approximately 87 kDa (predicted molecular weight: 85 kDa).
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.
  • 追加情報Is unsuitable for ICC.
  • ターゲット情報

    • 機能Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.
    • 組織特異性Brain and testis.
    • 関連疾患Note=Defects in BRAF are found in a wide range of cancers.
      Defects in BRAF may be a cause of colorectal cancer (CRC) [MIM:114500].
      Defects in BRAF are involved in lung cancer (LNCR) [MIM:211980].
      Defects in BRAF are involved in non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss.
      Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
      Defects in BRAF are the cause of Noonan syndrome type 7 (NS7) [MIM:613706]. Noonan syndrome is a disorder characterized by facial dysmorphic features such as hypertelorism, a downward eyeslant and low-set posteriorly rotated ears. Other features can include short stature, a short neck with webbing or redundancy of skin, cardiac anomalies, deafness, motor delay and variable intellectual deficits.
      Defects in BRAF are the cause of LEOPARD syndrome type 3 (LEOPARD3) [MIM:613707]. LEOPARD3 is a disorder characterized by lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and sensorineural deafness.
      Note=A chromosomal aberration involving BRAF is found in pilocytic astrocytomas. A tandem duplication of 2 Mb at 7q34 leads to the expression of a KIAA1549-BRAF fusion protein with a constitutive kinase activity and inducing cell transformation.
    • 配列類似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 protein kinase domain.
      Contains 1 RBD (Ras-binding) domain.
    • 細胞内局在Nucleus. Cytoplasm. Cell membrane. Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.
    • Information by UniProt
    • 参照データベース
    • 別名
      • FLJ95109 antibody
      • 94 kDa B raf protein antibody
      • B raf 1 antibody
      • B Raf proto oncogene serine threonine protein kinase antibody
      • B Raf proto oncogene, serine/threonine kinase antibody
      • B RAF1 antibody
      • B-Raf proto-oncogene serine/threonine-protein kinase (p94) antibody
      • BRAF 1 antibody
      • BRAF antibody
      • BRAF_HUMAN antibody
      • BRAF1 antibody
      • cRmil antibody
      • MGC126806 antibody
      • MGC138284 antibody
      • Murine sarcoma viral (v-raf) oncogene homolog B1 antibody
      • Murine sarcoma viral v raf oncogene homolog B1 antibody
      • NS7 antibody
      • Oncogen BRAF antibody
      • oncogene BRAF1 antibody
      • p94 antibody
      • Proto-oncogene B-Raf antibody
      • Proto-oncogene c-Rmil antibody
      • RAFB 1 antibody
      • RAFB1 antibody
      • RMIL antibody
      • Serine/threonine-protein kinase B-raf antibody
      • v raf murine sarcoma viral oncogene homolog B antibody
      • v raf murine sarcoma viral oncogene homolog B1 antibody
      • v-Raf murine sarcoma viral oncogene homolog B1 antibody
      see all

    Anti-B Raf antibody [EP152Y] 画像

    • This image shows paraffin embedded human prostate cancer tissue sample stained with ab33899 at 1/250 dilution.
    • Overlay histogram showing SH-SY5Y cells stained with ab33899 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab33899, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • ab33899 staining B Raf cells from human prostate tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were formaldehyde fixed and permeabilized in PBS-Tween 20 prior to blocking in 70% serum for 10 minutes at 25°C. The primary antibody was diluted 1/250 and incubated with the sample for 1 hour at 25°C. A biotin conjugated goat polyclonal to mouse Ig was used as the secondary.

      See Abreview

    • Anti-B Raf antibody [EP152Y] (ab33899) at 1/5000 dilution + HeLa cell lysate

      Predicted band size : 85 kDa
      Observed band size : 87 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-B Raf antibody [EP152Y] (ab33899) at 1/1000 dilution

      Lane 1 : Lysate prepared from mouse brain
      Lane 2 : Lysate prepared from mouse heart
      Lane 3 : Lysate prepared from mouse kidney
      Lane 4 : Lysate prepared from mouse spleen
      Lane 5 : Lysate prepared from rat brain
      Lane 6 : Lysate prepared from rat heart
      Lane 7 : Lysate prepared from rat kidney
      Lane 8 : Lysate prepared from rat spleen


      Predicted band size : 85 kDa


      Exposure time : 3 minutes

    Anti-B Raf antibody [EP152Y] (ab33899) 使用論文

    This product has been referenced in:
    • Brown WS  et al. B-Raf regulation of integrin a4ß1-mediated resistance to shear stress through changes in cell spreading and cytoskeletal association in T cells. J Biol Chem 289:23141-53 (2014). WB, ICC/IF ; Human . Read more (PubMed: 24936068) »
    • Hirschi B & Kolligs FT Alternative splicing of BRAF transcripts and characterization of C-terminally truncated B-Raf isoforms in colorectal cancer. Int J Cancer N/A:N/A (2013). WB ; Human . Read more (PubMed: 23354951) »

    See all 6 Publications for this product

    Product Wall

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

    Read More
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Methanol
    Permeabilization No
    Username

    Dr. Kirk McManus

    Verified customer

    投稿 Sep 05 2012

    Unfortunately shipment of this order is being held up by our accounts team.

    Our accounting department can be contacted by email at mailto:us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corne...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (melanoma cell lines)
    Loading amount 50 µg
    Specification melanoma cell lines
    Gel Running Conditions Reduced Denaturing (10% gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    投稿 Jun 02 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Prostate)
    Specification Prostate
    Fixative Formaldehyde
    Antigen retrieval step Other
    Permeabilization Yes - PBS-Tween20
    Blocking step methanol + hydrogen peroxide as blocking agent for 10 minute(s) · Concentration: 70% · Temperature: 25°C
    Username

    Dr. Serdar BALCI

    Verified customer

    投稿 Nov 11 2009

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (saggital sections of cerebellum and cortex)
    Specification saggital sections of cerebellum and cortex
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
    Permeabilization No
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
    Username

    Mr. Carl Hobbs

    Verified customer

    投稿 Feb 03 2009

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (adult brain)
    Specification adult brain
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid Hier, pH6
    Permeabilization No
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
    Username

    Mr. Carl Hobbs

    Verified customer

    投稿 Feb 02 2009

    Thank you for your enquiry. I have been in touch with the lab and I can tell you that this antibody has NOT been tested for its capacity to differentiate between wild type and variant (V600E) BRAF. I could not find literature relating to the varian...

    Read More
    Application Western blot
    Sample Mouse Tissue lysate - whole (brain, liver, skin from Tg animal expressing Human)
    Loading amount 100 µg
    Specification brain, liver, skin from Tg animal expressing Human
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    投稿 Oct 02 2006

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Glioma cell line)
    Loading amount 50 µg
    Specification Glioma cell line
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    投稿 Aug 24 2006

    1-10 of 12 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"