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The details of the immunogen for this antibody are not available.
ab25356 stains the following percentages of surface immunoglobulin (sIg) positive cells in porcine lymphoid tissues: 11-15% in peripheral blood; 22-23% in mesenteric lymph nodes; 79-87% in ileal Peyer’s patches (ILPP); 6-17 in spleen; and 0% in thymus. While identification of the antigen(s) that are found predominantly on ILPP B cells and which are recognized by ab25356 is speculative, the antibody appears to react with an immature population of B cells in the follicles of ILPPs and with a subpopulation of s Ig low B cells in lymph nodes.
Our Abpromise guarantee covers the use of ab25356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.2µg for 106 cells. ab154448-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.|
|IHC-Fr||Use at an assay dependent concentration.|
ab25356 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"