特異性The specificity of this antibody is indeterminate. It stains the following percentages of surface immunoglobulin (sIg) positive cells in porcine lymphoid tissues: 11-15% in peripheral blood; 22-23% in mesenteric lymph nodes; 79-87% in ileal Peyer's patches (ILPP); 6-17 in spleen; and 0% in thymus. While identification of the antigen(s) that are found predominantly on ILPP B cells and which are recognized by this is speculative, the antibody appears to react with an immature population of B cells in the follicles of ILPPs and with a subpopulation of sIg low B cells in lymph nodes.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 1µg for 106 cells.
ab91546 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration.
関連性B cells are lymphocytes that are produced in the bone marrow and require bone marrow stromal cells and their cytokines for maturation. During its development, each B cell becomes genetically programmed through a series of gene splicing reactions to produce an antibody molecule with a unique specificity.