製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to AZI1
  • アプリケーション
    適用あり: WB, IP, ICC, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region between amino acids 1-50 of Human AZI1 (EAW89632.1).

  • ポジティブ・コントロール
    • HeLa and 293T whole cell lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab99379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 122 kDa.
IP Use at 2-5 µg/mg of lysate.
ICC 1/100 - 1/500.

Formaldehyde fixation with Triton-X 100 permeabilization is recommended.

ICC/IF 1/100 - 1/500.

ターゲット情報

  • 機能
    May play a role in spermatogenesis.
  • 配列類似性
    Contains 1 IQ domain.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5 azacytidine induced 1 antibody
    • 5-azacytidine-induced protein 1 antibody
    • AZ1 antibody
    • Azi antibody
    • Azi1 antibody
    • AZI1_HUMAN antibody
    • Centrosomal protein 131 kDa antibody
    • Centrosomal protein of 131 kDa antibody
    • Cep131 antibody
    • KIAA1118 antibody
    • OTTMUSP00000004498 antibody
    • Pre-acrosome localization protein 1 antibody
    • RP23 37J21.1 antibody
    see all

Anti-AZI1 antibody 画像

  • Immunocytochemistry/Immunofluorescence analysis of NBF-fixed asynchronous HeLa cells labelling AZI1 with ab99379 at 1/200. A red-fluorescent goat anti-rabbit IgG (1/100) was used as the secondary antibody.
  • All lanes : Anti-AZI1 antibody (ab99379) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 122 kDa


    Exposure time : 30 seconds
  • Detection of AZI1 by Western Blot of Immunprecipitate.
    ab99379 at 1µg/ml staining AZI1 in HeLa whole cell lysate immunoprecipitated using ab99379 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 3 seconds.

Anti-AZI1 antibody (ab99379) 使用論文

This product has been referenced in:
  • Li X  et al. USP9X regulates centrosome duplication and promotes breast carcinogenesis. Nat Commun 8:14866 (2017). WB, IP . Read more (PubMed: 28361952) »
  • Airik R  et al. SDCCAG8 Interacts with RAB Effector Proteins RABEP2 and ERC1 and Is Required for Hedgehog Signaling. PLoS One 11:e0156081 (2016). Read more (PubMed: 27224062) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (U2OS cells)
Total protein in input
1000 µg
Immuno-precipitation step
Protein G
Specification
U2OS cells
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Abcam user community

Verified customer

投稿 Mar 15 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Different human tissue culture cell lines)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris Nupage)
Loading amount
40 µg
Specification
Different human tissue culture cell lines
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

投稿 Jan 18 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - other (MEFs and mouse brain lysate)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris Nupage)
Loading amount
40 µg
Specification
MEFs and mouse brain lysate
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jan 18 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS cells)
Loading amount
50 µg
Specification
U2OS cells
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

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Verified customer

投稿 Oct 10 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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