Anti-Axons of the Drosophila CNS 抗体 [BP102] (ab12455)

製品の概要

  • 製品名Anti-Axons of the Drosophila CNS antibody [BP102]
  • 製品の詳細
    Mouse monoclonal [BP102] to Axons of the Drosophila CNS
  • アプリケーション適用あり: IHC-Fr, IHC-FoFr, ICC, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Drosophila melanogaster
  • 免疫原

    Other Immunogen Type corresponding to Axons of the Drosophila CNS.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度IgG fraction
  • ポリ/モノモノクローナル
  • クローン名BP102
  • ミエローマNS1
  • アイソタイプIgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab12455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/1000.
IHC-FoFr 1/1000.
ICC 1/1000.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 別名
    • BP 102 Axons antibody
    • BP102 Axons antibody
    • Drosophila CNS axons antibody

Anti-Axons of the Drosophila CNS antibody [BP102] 画像

  • ab12455 used in immunofluorescence at 1/1000 on Drosophila embryos. The protocol used is available in the Neuroscience Abwire in the protocols section.

    Briefly, embryos were fixed with PBS, formaldehyde and n-heptane. Embryos were then dechorionated with 100% bleach and rehydrated with PBT (PBS with 0.3% Tritone added) before antibody staining.

  • ab12455 used in immunohistochemistry at 1/1000 on Drosophila embryos. The protocol used is available in the Neuroscience Abwire in the protocols section.

    Briefly, embryos were fixed with PBS, formaldehyde and n-heptane. Embryos were then dechorionated with 100% bleach and rehydrated with PBT (PBS with 0.3% Tritone added) before antibody staining. Visualisation was with alkaline phosphatase.

  • ab12455 staining axons in mouse brain tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with paraformaldehyde and blocking with a solution that contains 2% Block Ace, 0.05% Triton X-100 and PBS was performed at 250C for 1 hour. The sample was incubated with primary antibody (1/1000) in a dilution buffer containing 2% Block Ace, 0.05% Triton X-100 and PBS at 40C for 12 hours. Cyt3®-conjugated donkey polyclonal to mouse IgG was used as secondary antibody at 1/500 dilution.

    See Abreview

Anti-Axons of the Drosophila CNS antibody [BP102] (ab12455) 使用論文

This product has been referenced in:
  • Magalhães TR  et al. Transcriptional control in embryonic Drosophila midline guidance assessed through a whole genome approach. BMC Neurosci 8:59 (2007). Read more (PubMed: 17672901) »
  • Fraichard S  et al. Tenectin, a novel extracellular matrix protein expressed during Drosophila melanogaster embryonic development. Gene Expr Patterns 6:772-6 (2006). Read more (PubMed: 16510317) »

See all 16 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization No
Blocking step Blockace/0.05% Triton X-100/PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Apr 08 2010

Thank you for your enquiry. Unfortunately, we are unable to obtain the amino acid sequence for this product as the information is proprietary. I am afraid that all the information we have is already stated on the datasheet. I am sorry I can't b...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"