Anti-Axl 抗体 [MM0098-2N33] (ab89224)

製品の概要

  • 製品名Anti-Axl antibody [MM0098-2N33]
    Axl 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MM0098-2N33] to Axl
  • アプリケーション適用あり: IP, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Human, African Green Monkey
  • 免疫原

    Human recombinant Axl (extracellular region).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab89224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt 1/50 - 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能May function as a signal transducer between specific cell types of mesodermal origin. In case of filovirus infection, seems to function as a cell entry factor.
  • 組織特異性Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Adhesion related kinase antibody
    • AI323647 antibody
    • Ark antibody
    • Axl antibody
    • AXL oncogene antibody
    • AXL receptor tyrosine kinase antibody
    • AXL transforming gene antibody
    • AXL transforming sequence/gene antibody
    • EC 2.7.10.1 antibody
    • JTK11 antibody
    • Oncogene AXL antibody
    • Tyro7 antibody
    • Tyrosine protein kinase receptor UFO antibody
    • Tyrosine-protein kinase receptor UFO antibody
    • UFO antibody
    • UFO_HUMAN antibody
    see all

Anti-Axl antibody [MM0098-2N33] 画像

  • ab89224 used in Flow Cytometry.
    Cells were trypsinized for 1 minute at room temperature and wahed once in cold PBS. They were stained with ab89224 diluted 1/200 in 5% BSA/PBS for 45 minutes on ice, washed and incbuated with ab98794 at a 1/200 dilution. Cells were filtered and read in a FACScan using the FL1 sensor.
  • ab89224 staining Axl in african green monkey CV1 cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in 4% paraformaldehyde and then blocked using 5% serum for 1 hour at 22°C. Samples were then incubated with primary antibody at 1/100 for 16 hours at 4°C. The secondary antibody used was a donkey anti-mouse IgG conjugated to Alexa Fluor® 546 (red) used at a 1/400 dilution. DAPI was used to counterstain nuclei.

Anti-Axl antibody [MM0098-2N33] (ab89224) 使用論文

ab89224 has not yet been referenced specifically in any publications.

Product Wall

I can confirm that this product does not contain any azide. It only contains PBS.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (HeLa)
Specification HeLa
Preparation Cell harvesting/tissue preparation method: Cells were collected by trypsinization
Sample buffer: PBS
Fixation no fixation
Permeabilization No
Username

Mr. Nir Drayman

Verified customer

投稿 Nov 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample African Green Monkey Cell (CV1)
Specification CV1
Fixation no fixation
Permeabilization No
Username

Mr. Nir Drayman

Verified customer

投稿 May 28 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample African Green Monkey Cell lysate - whole cell (CV1 cells)
Total protein in input 1e+007 cells
Specification CV1 cells
Immuno-precipitation step Protein A/G
Username

Mr. Nir Drayman

Verified customer

投稿 May 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample African Green Monkey Cell (CV1)
Specification CV1
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Mr. Nir Drayman

Verified customer

投稿 Apr 21 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"