製品の概要

  • 製品名Anti-Axl antibody - C-terminal
    Axl 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Axl - C-terminal
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide from the C-terminal region of human Axl conjugated to KLH

  • ポジティブ・コントロール
    • SKBR3 cell lysate. Human breast carcinoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100.
WB 1/1000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).

ターゲット情報

  • 機能May function as a signal transducer between specific cell types of mesodermal origin. In case of filovirus infection, seems to function as a cell entry factor.
  • 組織特異性Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Adhesion related kinase antibody
    • AI323647 antibody
    • Ark antibody
    • Axl antibody
    • AXL oncogene antibody
    • AXL receptor tyrosine kinase antibody
    • AXL transforming gene antibody
    • AXL transforming sequence/gene antibody
    • EC 2.7.10.1 antibody
    • JTK11 antibody
    • Oncogene AXL antibody
    • Tyro7 antibody
    • Tyrosine protein kinase receptor UFO antibody
    • Tyrosine-protein kinase receptor UFO antibody
    • UFO antibody
    • UFO_HUMAN antibody
    see all

Anti-Axl antibody - C-terminal 画像

  • Anti-Axl antibody - C-terminal (ab72069) at 1/100 dilution + SKBR3 cell lysate at 12.5 µg

    Secondary
    HRP-anti-rabbit

    Predicted band size : 98 kDa
    Observed band size : 98 kDa
    Additional bands at : 25 kDa,37 kDa,50 kDa. We are unsure as to the identity of these extra bands.
  • ab72069 at 1/50 dilution, staining Axl in human breast carcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-Axl antibody - C-terminal (ab72069) 使用論文

This product has been referenced in:
  • Leconet W  et al. Preclinical validation of AXL receptor as a target for antibody-based pancreatic cancer immunotherapy. Oncogene N/A:N/A (2013). Read more (PubMed: 24240689) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MEFs)
Loading amount 10 µg
Specification MEFs
Treatment human Axl over expression
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Mr. Nir Drayman

Verified customer

投稿 Jan 05 2012

Application Immunocytochemistry/ Immunofluorescence
Sample African Green Monkey Cell (CV1 cells)
Specification CV1 cells
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Mr. Nir Drayman

Verified customer

投稿 Jan 31 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"