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RabMAb

Anti-Axin 2 抗体 [EPR2005(2)] (ab109307)

製品の概要

  • 製品名
    Anti-Axin 2 antibody [EPR2005(2)]
    Axin 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2005(2)] to Axin 2
  • アプリケーション
    適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Pig
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Axin 2 aa 50-150.

  • ポジティブ・コントロール
    • WB: C6, PC-12, NIH/3T3, MCF7, SW480, and PC3 cell lysates. IHC-P: Human lung carcinoma tissue. ICC/IF: LnCap cells.
  • 特記事項

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/150.
IHC-P 1/150.
WB 1/1000 - 1/2000. Detects a band of approximately 95 kDa (predicted molecular weight: 94 kDa).

ターゲット情報

  • 機能
    Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B.
  • 組織特異性
    Expressed in brain and lymphoblast.
  • 関連疾患
    Defects in AXIN2 are involved in colorectal cancer (CRC) [MIM:114500]. They appear to be specifically associated with defective mismatch repair.
    Defects in AXIN2 are the cause of oligodontia-colorectal cancer syndrome (ODCRCS) [MIM:608615]. Affected individuals manifest severe tooth agenesis and colorectal cancer or precancerous lesions of variable types.
  • 配列類似性
    Contains 1 DIX domain.
    Contains 1 RGS domain.
  • ドメイン
    The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2.
  • 翻訳後修飾
    Probably phosphorylated by GSK3B and dephosphorylated by PP2A.
    ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway.
    Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Axil antibody
    • Axin like protein antibody
    • Axin-2 antibody
    • Axin-like protein antibody
    • Axin2 antibody
    • AXIN2_HUMAN antibody
    • Axis inhibition protein 2 antibody
    • Conductin antibody
    • DKFZp781B0869 antibody
    • MGC10366 antibody
    • MGC126582 antibody
    see all

Anti-Axin 2 antibody [EPR2005(2)] 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling Axin 2 with unpurified ab109307 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • All lanes : Anti-Axin 2 antibody [EPR2005(2)] (ab109307) at 1/1000 dilution (unpurified)

    Lane 1 : SW480 cell lysate
    Lane 2 : C6 cell lysate
    Lane 3 : PC-12 cell lysate
    Lane 4 : NIH/3T3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 94 kDa
    Observed band size : 94 kDa

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Unpurified ab109307 staining Axin 2 in 293T cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 1% Triton X-100 and blocked with 3% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/50 in PBS + 3% BSA) for 16 hours. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-Axin 2 antibody [EPR2005(2)] (ab109307) at 1/1000 dilution (purified)

    Lane 1 : SW480 cell lysate
    Lane 2 : C6 cell lysate
    Lane 3 : PC-12 cell lysate
    Lane 4 : NIH/3T3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 94 kDa
    Observed band size : 94 kDa

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling Axin 2 with purified ab109307 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • All lanes : Anti-Axin 2 antibody [EPR2005(2)] (ab109307) at 1/1000 dilution (unpurified)

    Lane 1 : MCF7 cell lysates
    Lane 2 : SW480 cell lysates
    Lane 3 : PC3 cell lysates

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 94 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
  • Immunocytochemistry/Immunofluorescence analysis of LnCap cells labelling Axin 2 with unpurified ab109307 at 1/150. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Control: primary antibody (1/150) and secondary antibody, ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

  • Immunocytochemistry/Immunofluorescence analysis of LnCap cells labelling Axin 2 with purified ab109307 at 1/150. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Control: primary antibody (1/150) and secondary antibody, ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

Anti-Axin 2 antibody [EPR2005(2)] (ab109307) 使用論文

ab109307 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (MDA-MB-231 breast cancer xenograft)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization
No
Specification
MDA-MB-231 breast cancer xenograft
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Dec 14 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Sample
Human Cell (293T)
Specification
293T
Permeabilization
Yes - Triton 1%
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Nov 20 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH6
Sample
Mouse Tissue sections (MOUSE EMBRYO 20DPC)
Specification
MOUSE EMBRYO 20DPC
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Sep 09 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Pig Cell lysate - whole cell (PAE cell line (Pig Aortic Endothelial))
Loading amount
70 µg
Specification
PAE cell line (Pig Aortic Endothelial)
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Raul Peña

Verified customer

投稿 Dec 17 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Embryonic Stem Cells)
Specification
Embryonic Stem Cells
Fixative
Paraformaldehyde
Permeabilization
Yes - PBS 0,3% triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Mrs. Elisa Pedone

Verified customer

投稿 Nov 27 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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