Anti-Axin 2 抗体 [EPR2005(2)] (ab109307)
製品の概要
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製品名Anti-Axin 2 antibody [EPR2005(2)]
Axin 2 一次抗体 製品一覧 -
製品の詳細Rabbit monoclonal [EPR2005(2)] to Axin 2
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由来種Rabbit
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アプリケーション適用あり: ICC/IF, IHC-P, WBmore details
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種交差性交差種: Mouse, Rat, Human, Pig
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免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Axin 2 aa 50-150.
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ポジティブ･コントロール
- WB: C6, PC-12, NIH/3T3, MCF7, SW480, and PC3 cell lysates. IHC-P: Human lung carcinoma tissue. ICC/IF: LnCap cells.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
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バッファーpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading... -
精製度Protein A purified
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ポリ/モノモノクローナル
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クローン名EPR2005(2)
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アイソタイプIgG
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研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab109307 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | 1/150. | |
| IHC-P | 1/150. | |
| WB | 1/1000 - 1/2000. Detects a band of approximately 95 kDa (predicted molecular weight: 94 kDa). |
ターゲット情報
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機能Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B.
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組織特異性Expressed in brain and lymphoblast.
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関連疾患Defects in AXIN2 are involved in colorectal cancer (CRC) [MIM:114500]. They appear to be specifically associated with defective mismatch repair.
Defects in AXIN2 are the cause of oligodontia-colorectal cancer syndrome (ODCRCS) [MIM:608615]. Affected individuals manifest severe tooth agenesis and colorectal cancer or precancerous lesions of variable types. -
配列類似性Contains 1 DIX domain.
Contains 1 RGS domain. -
ドメインThe tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2.
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翻訳後修飾Probably phosphorylated by GSK3B and dephosphorylated by PP2A.
ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway.
Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription. -
細胞内局在Cytoplasm.
- Information by UniProt
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参照データベース
- Entrez Gene: 8313 Human
- Entrez Gene: 12006 Mouse
- Entrez Gene: 29134 Rat
- Omim: 604025 Human
- SwissProt: Q9Y2T1 Human
- SwissProt: O88566 Mouse
- SwissProt: O70240 Rat
- Unigene: 156527 Human
see all -
別名
- Axil antibody
- Axin like protein antibody
- Axin-2 antibody
see all
画像
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)](http://a.abcam.com/ps/products/109/ab109307/Images/ab109307-239113-ab109307upihcp.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling Axin 2 with unpurified ab109307 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Western blot - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)](http://a.abcam.com/ps/products/109/ab109307/Images/ab109307-239111-ab109307upwb.jpg)
Western blot - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)All lanes : Anti-Axin 2 antibody [EPR2005(2)] (ab109307) at 1/1000 dilution (unpurified)
Lane 1 : SW480 cell lysate
Lane 2 : C6 cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : NIH/3T3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 94 kDa
Observed band size: 94 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)](http://a.abcam.com/ps/products/109/ab109307/Images/ab109307-237752-ab109307iccif.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)This image is courtesy of an anonymous AbreviewUnpurified ab109307 staining Axin 2 in 293T cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 1% Triton X-100 and blocked with 3% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/50 in PBS + 3% BSA) for 16 hours. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
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![Western blot - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)](http://a.abcam.com/ps/products/109/ab109307/Images/ab109307-239112-ab109307pwb.jpg)
Western blot - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)All lanes : Anti-Axin 2 antibody [EPR2005(2)] (ab109307) at 1/1000 dilution (purified)
Lane 1 : SW480 cell lysate
Lane 2 : C6 cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : NIH/3T3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 94 kDa
Observed band size: 94 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)](http://a.abcam.com/ps/products/109/ab109307/Images/ab109307-239114-ab109307pihcp.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling Axin 2 with purified ab109307 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Western blot - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)](http://a.abcam.com/ps/datasheet/images/109/ab109307/Axin-2-Primary-antibodies-ab109307-1.jpg)
Western blot - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)All lanes : Anti-Axin 2 antibody [EPR2005(2)] (ab109307) at 1/1000 dilution (unpurified)
Lane 1 : MCF7 cell lysates
Lane 2 : SW480 cell lysates
Lane 3 : PC3 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 94 kDa
Observed band size: 95 kDa (why is the actual band size different from the predicted?) -
![Immunocytochemistry/ Immunofluorescence - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)](http://a.abcam.com/ps/products/109/ab109307/Images/ab109307-239116-ab109307upiccif.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)Immunocytochemistry/Immunofluorescence analysis of LnCap cells labelling Axin 2 with unpurified ab109307 at 1/150. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
Control: primary antibody (1/150) and secondary antibody, ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).
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![Immunocytochemistry/ Immunofluorescence - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)](http://a.abcam.com/ps/products/109/ab109307/Images/ab109307-239117-ab109307piccif.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Axin 2 antibody [EPR2005(2)] (ab109307)Immunocytochemistry/Immunofluorescence analysis of LnCap cells labelling Axin 2 with purified ab109307 at 1/150. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
Control: primary antibody (1/150) and secondary antibody, ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).
プロトコール
参考文献
This product has been referenced in:
- Fan HB et al. CDX2 Stimulates the Proliferation of Porcine Intestinal Epithelial Cells by Activating the mTORC1 and Wnt/ß-Catenin Signaling Pathways. Int J Mol Sci 18:N/A (2017). Read more (PubMed: 29156556) »
- Wu X et al. Spatiotemporal Expression of Wnt/ß-catenin Signaling during Morphogenesis and Odontogenesis of Deciduous Molar in Miniature Pig. Int J Biol Sci 13:1082-1091 (2017). Read more (PubMed: 28924388) »
レビューと Q&A
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