製品の概要

  • 製品名Anti-Axin 2 antibody
    Axin 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Axin 2
  • 特異性Milk blocking not recommended
  • アプリケーション適用あり: IHC-P, IHC-Fr, ICC/IF, WB, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chicken
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Mouse Axin 2.

    (Peptide available as ab32196.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in: SW480, HCT 116, LS147T whole cell lysates; mouse colon tissue lysates and AXIN2 partial recombinant tagged protein

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 0.1 - 1 µg/ml.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Can be blocked with Mouse Axin 2 peptide (ab32196). Detects a band of approximately 94 kDa (predicted molecular weight: 94 kDa) in endogenous lysates. Ab32197 gave a positive signal against the tagged partial recombinant protein which has an expected molecular weight of 37 kDa.
Flow Cyt 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B.
  • 組織特異性Expressed in brain and lymphoblast.
  • 関連疾患Defects in AXIN2 are involved in colorectal cancer (CRC) [MIM:114500]. They appear to be specifically associated with defective mismatch repair.
    Defects in AXIN2 are the cause of oligodontia-colorectal cancer syndrome (ODCRCS) [MIM:608615]. Affected individuals manifest severe tooth agenesis and colorectal cancer or precancerous lesions of variable types.
  • 配列類似性Contains 1 DIX domain.
    Contains 1 RGS domain.
  • ドメインThe tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2.
  • 翻訳後修飾Probably phosphorylated by GSK3B and dephosphorylated by PP2A.
    ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway.
    Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Axil antibody
    • Axin like protein antibody
    • Axin-2 antibody
    • Axin-like protein antibody
    • Axin2 antibody
    • AXIN2_HUMAN antibody
    • Axis inhibition protein 2 antibody
    • Conductin antibody
    • DKFZp781B0869 antibody
    • MGC10366 antibody
    • MGC126582 antibody
    see all

Anti-Axin 2 antibody 画像

  • ab32197 staining Axin2 in mouse colon sections. Secondary antibody conjugated to Alexa-fluor ® 488 (please see Abreview for further details)

    See Abreview

  • IHC image of ab32197 stained area of normal tissue in a human colorectal cancer FFPE section. Section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30 seconds at 125°C. Section was incubated with ab32197 at a dilution of 1:500 for 1h at room temperature and detected using an HRP conjugated polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • All lanes : Anti-Axin 2 antibody (ab32197) at 1 µg/ml

    Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : Colon (Mouse) Tissue Lysate
    Lane 3 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
    Lane 4 : LS147T (Colon cancer cells) (Human) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 94 kDa
    Observed band size : 94 kDa
    Additional bands at : 32 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 16 minutes
  • Anti-Axin 2 antibody (ab32197) at 1 µg/ml + AXIN2 partial recombinant tagged protein at 0.1 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 94 kDa

    ab32197 gave a positive signal against the tagged partial recombinant protein which has an expected molecular weight of 37 kDa.

Anti-Axin 2 antibody (ab32197) 使用論文

This product has been referenced in:
  • Ng OH  et al. Deregulated WNT signaling in childhood T-cell acute lymphoblastic leukemia. Blood Cancer J 4:e192 (2014). WB ; Human . Read more (PubMed: 24632884) »
  • Winkler T  et al. Wnt signaling activates Shh signaling in early postnatal intervertebral discs, and re-activates Shh signaling in old discs in the mouse. PLoS One 9:e98444 (2014). IHC-Fr ; Mouse . Read more (PubMed: 24892825) »

See all 17 Publications for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Tissue lysate - whole (colon cancer colitis induced)
Specification colon cancer colitis induced
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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投稿 Jul 25 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (HCT116 colorectal adenocarcinoma)
Specification HCT116 colorectal adenocarcinoma
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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投稿 Jul 25 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Colon)
Specification Colon
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM Citrate
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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投稿 Feb 13 2013

Thank you for your enquiry which has been forwarded to the scientific support team. I can confirm that there was indeed an error and the datasheet has been amended by our datasheets team. I sincerely apologise for any confusion.

The quality ...

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Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (mouse E10.5 embryo)
Specification mouse E10.5 embryo
Fixative Paraformaldehyde
Permeabilization Yes - PBST (PBS/0.5%v/v TritonX100 (Sigma)
Blocking step NOVOCASTRA protein block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
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投稿 Jun 08 2012

Thank you very much for these precisions.

I would recommend to use 5% BSA as blocking agent. This is what is used in our lab to characterise the antibodies. We also use this blocking solution to dilute both primary and secondary antibodies. <...

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Thanks for sending along the sequence.

Unfortunately the alignment results show there is no sequence similarity between the peptide immunogen used to produce ab32197 and canine Axin2. So I don't think this antibody will cross react with your ...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry free floating
Sample Chicken Tissue sections (HH 24 trunk section)
Specification HH 24 trunk section
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投稿 Nov 10 2011

I am unable to send you both antibodies as replacements but what I can do is send you one as a free replacement and also, as whichever one you receive has not been tested in IHC, I can also send a testing discount code. What that means is that you test...

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