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Synthetic peptide: FEEKIIGKVEKVD, corresponding to C terminal amino acids 850-862 of Human AXIN1.
Our Abpromise guarantee covers the use of ab4157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Can be blocked with Human Axin 1 peptide (ab23000). No signal obtained yet but low background observed in Human Brain, Human Pancreas, MOLT-4 and HeLa lysates at up to 2.0µg/ml.|
|IHC-P||Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab4157 has not yet been referenced specifically in any publications.