製品の概要

  • 製品名Anti-avrA antibody
  • 製品の詳細
    Mouse polyclonal to avrA
  • アプリケーション適用あり: WBmore details
  • 種交差性
    Reacts with Salmonella typhimurium.
  • 免疫原

    Vector coding for a partial recombinant fusion protein, corresponding to amino acids 160-259 of Salmonella typhimurium avrA, [Accession number AAB83970.1] Target sequence used to make the antibody: AALEREQLPD CYFAMVELDI QRSSSECGIF SLALAKKLQL EFMNLVKIHE DNICERLCGE EPFLPSDKAD RYLPVSFYKH TQGAQRLNEY VEANPAAGSS.

  • 特記事項


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • 精製度Whole antiserum
  • 一次抗体 備考This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab52929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 34 kDa. This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 関連性AvrA, a secreted virulence-associated effector protein, and is a member of the YopJ/Avr family of proteins. It is found in most Salmonella species. It has been reported that the AvrA protein from Salmonella typhimurium is an effector molecule that inhibits activation of the key proinflammatory NF-kB transcription factor and augments apoptosis in human epithelial cells. This activity is similar but mechanistically distinct from that described for YopJ, an AvrA homolog expressed by the bacterial pathogen Yersinia.
  • 細胞内局在Secreted
  • 別名
    • Avirulence protein A antibody
    • Putative inner membrane protein antibody

Anti-avrA antibody 画像

  • All lanes : Anti-avrA antibody (ab52929) at 1/1000 dilution

    Lane 1 : a total protein extract from E coli with 50ng of a tagged fusion protein of an irrelevant antigen
    Lane 2 : a total protein extract from E coli with 50ng of the antigen (tagged fusion protein)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit anti-mouse IgG + IgM (H+L), HRP conjugated, at 1/5000 dilution

    Predicted band size : 34 kDa

Anti-avrA antibody (ab52929) 使用論文

ab52929 has not yet been referenced specifically in any publications.

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