アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。
Autophagy/Cytotoxicity Dual Staining Kit (ab133075) provides a convenient tool for studying the regulation of autophagy and cytotoxicity at the cellular level. The kit employs monodansylcadaverine (MDC), a fluorescent compound that is incorporated into multilamellar bodies by both an ion trapping mechanism and the interaction with membrane lipids, as a probe for detection of autophagic vacuoles in cultured cells. Propidium iodide (PI) is used as a marker of cell death. Tamoxifen, a known inducer of autophagy, is included as a positive control. This kit provides sufficient reagent to effectively treat/stain 960 individual wells of cells when utilized in a 96-well plate format. Lower density plates will still require approximately the same amount of reagent on a per plate basis. Therefore, up to 10 plates worth of cells can be examined irrespective of the number of wells/plate (this is not the case for protocols that use non-adherent cells).
Autophagy is a critical cellular process that involves the degradation and digestion of intracellular components by the lysosome. This process not only enables cells to efficiently mobilize and recycle cellular constituents, but also prevents the accumulation of damaged organelles, misfolded proteins, and invading microorganisms.
Autophagy is a multi-step process that begins with the sequestration of cytoplasmic organelles and proteins. These cellular components are sequestered by a double membrane, forming an autophagosome. The autophagosome then fuses with a lysosome to form an autolysosome, where the cellular material is then degraded. Normal autophagy is essential for survival, differentiation, development, and homeostasis. Dysregulation of autophagy has been implicated in cancer, infection, aging, and degenerative diseases.
While autophagy most often acts to promote cell survival in response to stress, it can also promote cell death. The relationship between autophagy and apoptosis is complex. The two pathways share common stimuli and components, and can regulate the activity of each other. However, the specific factors and mechanisms that dictate the choice between autophagy and apoptosis remain unclear.
|Cell-Based Assay Buffer Tablet||5 tablets|
|Cell-Based Monodansylcadaverine||1 x 100µl|
|Cell-Based Propidium Iodide Solution||1 x 250µl|
|Cell-Based Tamoxifen (100 mM)||1 x 50µl|
Our Abpromise guarantee covers the use of ab133075 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
|FM||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"