Anti-Aurora B (phospho T232) 抗体 (ab115793)

製品の概要

  • 製品名Anti-Aurora B (phospho T232) antibody
    Aurora B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Aurora B (phospho T232)
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human, Pig
  • 免疫原

    Synthetic peptide corresponding to Human Aurora B. Synthetic peptide corresponding to a portion of human Aurora B phosphorylated at Thr232
    Database link: Q96GD4

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab115793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. ECL detection
IHC-P Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP.
  • 組織特異性High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
  • 関連疾患Note=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.
  • 配列類似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
  • 細胞内局在Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AIK2 antibody
    • AIM-1 antibody
    • AIM1 antibody
    • ARK-2 antibody
    • ARK2 antibody
    • AurB antibody
    • AURKB antibody
    • AURKB_HUMAN antibody
    • Aurora 1 antibody
    • Aurora and Ipl1 like midbody associated protein 1 antibody
    • Aurora kinase B antibody
    • Aurora related kinase 2 antibody
    • Aurora- and Ipl1-like midbody-associated protein 1 antibody
    • Aurora-B antibody
    • Aurora-related kinase 2 antibody
    • Aurora/IPL1 related kinase 2 antibody
    • Aurora/IPL1-related kinase 2 antibody
    • IPL1 antibody
    • PPP1R48 antibody
    • Protein phosphatase 1 regulatory subunit 48 antibody
    • Serine/theronine kinase 12 antibody
    • Serine/threonine protein kinase 12 antibody
    • Serine/threonine-protein kinase 12 antibody
    • Serine/threonine-protein kinase aurora-B antibody
    • STK-1 antibody
    • STK1 antibody
    • STK12 antibody
    • STK5 antibody
    see all

Anti-Aurora B (phospho T232) antibody 画像

  • Immunohistochemical analysis of paraffin embedded Human skin tissue labeling Aurora B (phospho T232) with ab115793 at 5 ug/ml.

     

     

  • Immunohistochemical analysis of paraffin embedded Human adrenal tissue labeling Aurora B (phospho T232) with ab115793 at 5 ug/ml.

     

     

Anti-Aurora B (phospho T232) antibody (ab115793) 使用論文

ab115793 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Sample Mouse Cell (NMuMG)
Specification NMuMG
Permeabilization Yes - PBS Tx100 0,3%
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Nov 20 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9
Sample Mouse Tissue sections (Mouse 14.5dpc)
Specification Mouse 14.5dpc
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Nov 15 2013

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (15% acrilamide)
Sample Mouse Cell lysate - whole cell (NmMG)
Specification NmMG
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Nov 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"