製品の概要

  • 製品名Anti-Aurora B antibody
    Aurora B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Aurora B
  • アプリケーション適用あり: WB, IP, ICC/IF, ICCmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 300 and the C-terminus (residue 344) of Human Aurora B (Swiss-Prot Q96GD4).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000 - 1/15000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
IP Use at 1-4 µg/mg of lysate.
ICC/IF Use at an assay dependent concentration. PubMed: 21844210
ICC 1/500 - 1/2000.

ターゲット情報

  • 機能May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Phosphorylates 'Ser-10' and 'Ser-28' of histone H3 during mitosis. Required for kinetochore localization of BUB1 and SGOL1. Interacts with INCENP.
  • 組織特異性High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
  • 関連疾患Note=Disruptive regulation of expression is a possibile mechanism of the perturbation of chromosomal integrity in cancer cells through its dominant-negative effect on cytokinesis.
  • 配列類似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
  • 細胞内局在Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AIK2 antibody
    • AIM-1 antibody
    • AIM1 antibody
    • ARK-2 antibody
    • ARK2 antibody
    • AurB antibody
    • AURKB antibody
    • AURKB_HUMAN antibody
    • Aurora 1 antibody
    • Aurora and Ipl1 like midbody associated protein 1 antibody
    • Aurora kinase B antibody
    • Aurora related kinase 2 antibody
    • Aurora- and Ipl1-like midbody-associated protein 1 antibody
    • Aurora-B antibody
    • Aurora-related kinase 2 antibody
    • Aurora/IPL1 related kinase 2 antibody
    • Aurora/IPL1-related kinase 2 antibody
    • IPL1 antibody
    • PPP1R48 antibody
    • Protein phosphatase 1 regulatory subunit 48 antibody
    • Serine/theronine kinase 12 antibody
    • Serine/threonine protein kinase 12 antibody
    • Serine/threonine-protein kinase 12 antibody
    • Serine/threonine-protein kinase aurora-B antibody
    • STK-1 antibody
    • STK1 antibody
    • STK12 antibody
    • STK5 antibody
    see all

Anti-Aurora B antibody 画像

  • Immunocytochemistry/Immunofluorescence analysis of asynchronous HeLa cells labelling Aurora B with ab70238 at 0.5µg/ml in A and at 2µg/ml in B. A FITC-conjugated anti-rabbit IgG was used as the secondary antibody in A. A Texas Red-conugated anti-rabbit IgG was used as the secondary antibody in B.
  • All lanes : Anti-Aurora B antibody (ab70238) at 1/10000 dilution

    Lane 1 : Whole cell lysate from non-adherent HeLa cells treated with nocodazole (20h)
    Lane 2 : Whole cell lysate from adherent HeLa cells treated with nocodazole (20h)
    Lane 3 : Whole cell lysate from non-adherent HeLa cells treated with nocodazole (20h)
    Lane 4 : Whole cell lysate from adherent HeLa cells treated with nocodazole (20h)

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
    Additional bands at : 28 kDa,36 kDa. We are unsure as to the identity of these extra bands.
    Detection time: 10 secs (lanes 1,2) or 30 secs (lanes 3,4)
  • All lanes : Anti-Aurora B antibody (ab70238) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate from an asynchronous culture
    Lane 2 : HeLa whole cell lysate treated with cycloheximide for 12 hours
    Lane 3 : HeLa whole cell lysatetreated with cycloheximide for 24 hours
    Lane 4 : HeLa whole cell lysate treated with cycloheximide for 36 hours
    Lane 5 : HeLa whole cell lysate treated with nocodazole for 20 hours

    Lysates/proteins at 100 µg per lane.


    Predicted band size : 39 kDa

Anti-Aurora B antibody (ab70238) 使用論文

This product has been referenced in:
  • Martz MK  et al. Leukemia-associated RhoGEF (LARG) is a novel RhoGEF in cytokinesis and required for the proper completion of abscission. Mol Biol Cell 24:2785-94 (2013). Read more (PubMed: 23885121) »
  • Wang E  et al. Aurora B dynamics at centromeres create a diffusion-based phosphorylation gradient. J Cell Biol 194:539-49 (2011). ICC/IF ; Human . Read more (PubMed: 21844210) »

See all 2 Publications for this product

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